{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Li H"],"funding":["National Science Foundation (NSF)","NIGMS NIH HHS","National Institute of General Medical Sciences (NIGMS)"],"pagination":["29-49"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6363205"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(1)"],"pubmed_abstract":["Visualizing diverse anatomical and functional traits that span many spatial scales with high spatio-temporal resolution provides insights into the fundamentals of living organisms. Light-field microscopy (LFM) has recently emerged as a scanning-free, scalable method that allows for high-speed, volumetric functional brain imaging. Given those promising applications at the tissue level, at its other extreme, this highly-scalable approach holds great potential for observing structures and dynamics in single-cell specimens. However, the challenge remains for current LFM to achieve a subcellular level, near-diffraction-limited 3D spatial resolution. Here, we report high-resolution LFM (HR-LFM) for live-cell imaging with a resolution of 300-700 nm in all three dimensions, an imaging depth of several micrometers, and a volume acquisition time of milliseconds. We demonstrate the technique by imaging various cellular dynamics and structures and tracking single particles. The method may advance LFM as a particularly useful tool for understanding biological systems at multiple spatio-temporal levels."],"journal":["Biomedical optics express"],"pubmed_title":["Fast, volumetric live-cell imaging using high-resolution light-field microscopy."],"pmcid":["PMC6363205"],"funding_grant_id":["R01GM084251","R35 GM124898","R35GM124846","R01 GM084251","R35 GM124846","EFMA1830941","CBET1604565"],"pubmed_authors":["Li W","Takamaru KI","Li H","Altshuller Y","Jia S","French JB","Guo C","Kim-Holzapfel D","Meng Y","Frohman MA","Schroeder B","Liu W"],"additional_accession":[]},"is_claimable":false,"name":"Fast, volumetric live-cell imaging using high-resolution light-field microscopy.","description":"Visualizing diverse anatomical and functional traits that span many spatial scales with high spatio-temporal resolution provides insights into the fundamentals of living organisms. Light-field microscopy (LFM) has recently emerged as a scanning-free, scalable method that allows for high-speed, volumetric functional brain imaging. Given those promising applications at the tissue level, at its other extreme, this highly-scalable approach holds great potential for observing structures and dynamics in single-cell specimens. However, the challenge remains for current LFM to achieve a subcellular level, near-diffraction-limited 3D spatial resolution. Here, we report high-resolution LFM (HR-LFM) for live-cell imaging with a resolution of 300-700 nm in all three dimensions, an imaging depth of several micrometers, and a volume acquisition time of milliseconds. We demonstrate the technique by imaging various cellular dynamics and structures and tracking single particles. The method may advance LFM as a particularly useful tool for understanding biological systems at multiple spatio-temporal levels.","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Jan","modification":"2026-07-11T03:18:15.31Z","creation":"2026-07-11T03:09:47.043Z"},"accession":"S-EPMC6363205","cross_references":{"pubmed":["30775081"],"doi":["10.1364/BOE.10.000029","10.1364/boe.10.000029"]}}