biostudies-literatureOzdemir OScientific Research Projects Coordination Unit of Istanbul UniversityTUBITAKIstanbul Development Agencye0211917https://www.ebi.ac.uk/biostudies/studies/S-EPMC6368378biostudies-literatureUnknown14(2)Genetic generalized epilepsies (GGE) are genetically determined, as their name implies and they are clinically characterized by generalized seizures involving both sides of the brain in the absence of detectable brain lesions or other known causes. GGEs are yet complex and are influenced by many different genetic and environmental factors. Methylation specific epigenetic marks are one of the players of the complex epileptogenesis scenario leading to GGE. In this study, we have set out to perform genome-wide methylation profiling to analyze GGE trios each consisting of an affected parent-offspring couple along with an unaffected parent. We have developed a novel scoring scheme within trios to categorize each locus analyzed as hypo or hypermethylated. This stringent approach classified diffePloS oneIdentification of epilepsy related pathways using genome-wide DNA methylation measures: A trio-based approach.PMC6368378TR10/15/YNK/0093ONAP-11021IntenC-109S218Sezerman OUOzbek UEgemen EBaykan BUgur Iseri SABebek NOzdemir OfalseIdentification of epilepsy related pathways using genome-wide DNA methylation measures: A trio-based approach.Genetic generalized epilepsies (GGE) are genetically determined, as their name implies and they are clinically characterized by generalized seizures involving both sides of the brain in the absence of detectable brain lesions or other known causes. GGEs are yet complex and are influenced by many different genetic and environmental factors. Methylation specific epigenetic marks are one of the players of the complex epileptogenesis scenario leading to GGE. In this study, we have set out to perform genome-wide methylation profiling to analyze GGE trios each consisting of an affected parent-offspring couple along with an unaffected parent. We have developed a novel scoring scheme within trios to categorize each locus analyzed as hypo or hypermethylated. This stringent approach classified diffe2019-01-01T00:00:00Z20192026-05-03T17:12:54.191Z2019-06-06T20:50:49ZS-EPMC63683783073554110.1371/journal.pone.0211917