biostudies-literatureHerath HMPDUniversity of MelbourneSuper Science Initiative and the Peter MacCallum Cancer Centre FoundationMedicines for Malaria Venture (MMV)Yourgene Bioscience and The University of MelbourneAustralian Government’s Education InvestmentAustralian Research Council72-79https://www.ebi.ac.uk/biostudies/studies/S-EPMC6369141biostudies-literatureUnknown9Due to the widespread occurrence and spread of anthelmintic resistance, there is a need to develop new drugs against resistant parasitic nematodes of livestock animals. The Nobel Prize-winning discovery and development of the anti-parasitic drugs avermectin and artemisinin has renewed the interest in exploring natural products as anthelmintics. In the present study, we screened 7500 plant extracts for in vitro-activity against the barber's pole worm, Haemonchus contortus, a highly significant pathogen of ruminants. The anthelmintic extracts from two plants, Cryptocarya novoguineensis and Piper methysticum, were fractionated by high-performance liquid chromatography (HPLC). Subsequently, compounds were purified from fractions with significant biological activity. Four α-pyrones, namely goniInternational journal for parasitology. Drugs and drug resistanceSelected α-pyrones from the plants Cryptocarya novoguineensis (Lauraceae) and Piper methysticum (Piperaceae) with activity against Haemonchus contortus in vitro.PMC63691410707258Preston SAddison RSJabbar ADavis RAChang BCHGasser RBRali TWang THofmann AKoehler AVHerath HMPDHayes SGarcia-Bustos JfalseSelected α-pyrones from the plants Cryptocarya novoguineensis (Lauraceae) and Piper methysticum (Piperaceae) with activity against Haemonchus contortus in vitro.Due to the widespread occurrence and spread of anthelmintic resistance, there is a need to develop new drugs against resistant parasitic nematodes of livestock animals. The Nobel Prize-winning discovery and development of the anti-parasitic drugs avermectin and artemisinin has renewed the interest in exploring natural products as anthelmintics. In the present study, we screened 7500 plant extracts for in vitro-activity against the barber's pole worm, Haemonchus contortus, a highly significant pathogen of ruminants. The anthelmintic extracts from two plants, Cryptocarya novoguineensis and Piper methysticum, were fractionated by high-performance liquid chromatography (HPLC). Subsequently, compounds were purified from fractions with significant biological activity. Four α-pyrones, namely goni2019-01-01T00:00:00Z2019 Apr2025-06-01T00:36:29.894Z2025-06-01T00:36:29.894ZS-EPMC63691413073907810.1016/j.ijpddr.2018.12.006