biostudies-literatureUnknown294(6)Bachu MThe variant histone H3.3 is incorporated into the genome in a transcription-dependent manner. This histone is thus thought to play a role in epigenetic regulation. However, our understanding of how H3.3 controls gene expression and epigenome landscape has remained incomplete. This is partly because precise localization of H3.3 in the genome has been difficult to decipher particularly for cells <i>in vivo</i> To circumvent this difficulty, we generated knockin mice, by homologous recombination, to replace both of the two H3.3 loci (<i>H3f3a</i> and <i>H3f3b</i>) with the hemagglutinin-tagged H3.3 cDNA cassette, which also contained a GFP gene. We show here that the hemagglutinin-tagged H3.3 and GFP are expressed in the majority of cells in all adult tissues tested. ChIP-seq data, combined wThe Journal of biological chemistry1904-1914https://www.ebi.ac.uk/biostudies/studies/S-EPMC6369307biostudies-literatureA versatile mouse model of epitope-tagged histone H3.3 to study epigenome dynamics.PMC6369307Narain AGhosh ANehru VKavarthapu ROzato KSarai NGhosh SBDufau MLBachu MChen CTamura TfalseA versatile mouse model of epitope-tagged histone H3.3 to study epigenome dynamics.The variant histone H3.3 is incorporated into the genome in a transcription-dependent manner. This histone is thus thought to play a role in epigenetic regulation. However, our understanding of how H3.3 controls gene expression and epigenome landscape has remained incomplete. This is partly because precise localization of H3.3 in the genome has been difficult to decipher particularly for cells <i>in vivo</i> To circumvent this difficulty, we generated knockin mice, by homologous recombination, to replace both of the two H3.3 loci (<i>H3f3a</i> and <i>H3f3b</i>) with the hemagglutinin-tagged H3.3 cDNA cassette, which also contained a GFP gene. We show here that the hemagglutinin-tagged H3.3 and GFP are expressed in the majority of cells in all adult tissues tested. ChIP-seq data, combined w2019-01-01T00:00:00Z2019 Feb2026-05-01T04:43:08.018Z2020-11-07T11:46:41ZS-EPMC63693073055211610.1074/jbc.RA118.00555010.1074/jbc.ra118.005550