biostudies-literatureRicciotti ENCATS NIH HHSNational Heart, Lung, and Blood InstituteNational Institutes of Healthe0207503https://www.ebi.ac.uk/biostudies/studies/S-EPMC6370185biostudies-literatureUnknown14(2)The cardiovascular biology of proton radiotherapy is not well understood. We aimed to compare the genomic dose-response to proton and gamma radiation of the mouse aorta to assess whether their vascular effects may diverge. We performed comparative RNA sequencing of the aorta following (4 hrs) total-body proton and gamma irradiation (0.5-200 cGy whole body dose, 10 dose levels) of conscious mice. A trend analysis identified genes that showed a dose response. While fewer genes were dose-responsive to proton than gamma radiation (29 vs. 194 genes; q-value ≤ 0.1), the magnitude of the effect was greater. Highly responsive genes were enriched for radiation response pathways (DNA damage, apoptosis, cellular stress and inflammation; p-value ≤ 0.01). Gamma, but not proton radiation induced additioPloS oneDistinct vascular genomic response of proton and gamma radiation-A pilot investigation.PMC6370185UL1TR000003UL1 TR000003HL117798Krigsfeld GSGrosser TKiliti AJKennedy ARSanzari JKRicciotti EGrant GRSarantopoulou DfalseDistinct vascular genomic response of proton and gamma radiation-A pilot investigation.The cardiovascular biology of proton radiotherapy is not well understood. We aimed to compare the genomic dose-response to proton and gamma radiation of the mouse aorta to assess whether their vascular effects may diverge. We performed comparative RNA sequencing of the aorta following (4 hrs) total-body proton and gamma irradiation (0.5-200 cGy whole body dose, 10 dose levels) of conscious mice. A trend analysis identified genes that showed a dose response. While fewer genes were dose-responsive to proton than gamma radiation (29 vs. 194 genes; q-value ≤ 0.1), the magnitude of the effect was greater. Highly responsive genes were enriched for radiation response pathways (DNA damage, apoptosis, cellular stress and inflammation; p-value ≤ 0.01). Gamma, but not proton radiation induced additio2019-01-01T00:00:00Z20192026-04-08T03:36:46.364Z2019-06-06T20:51:06ZS-EPMC63701853074263010.1371/journal.pone.0207503