biostudies-literatureSally MBNCATS NIH HHS208-216https://www.ebi.ac.uk/biostudies/studies/S-EPMC6374499biostudies-literatureUnknown2(4)<h4>Introduction</h4>A majority of transplanted organs come from donors after brain death (BD). Renal grafts from these donors have higher delayed graft function and lower long-term survival rates compared to living donors. We designed a novel porcine BD model to better delineate the incompletely understood inflammatory response to BD, hypothesizing that adhesion molecule pathways would be upregulated in BD.<h4>Methods</h4>Animals were anesthetized and instrumented with monitors and a balloon catheter, then randomized to control and BD groups. BD was induced by inflating the balloon catheter and animals were maintained for 6 hours. RNA was extracted from kidneys, and gene expression pattern was determined.<h4>Results</h4>In total, 902 gene pairs were differently expressed between groups. EJournal of clinical and translational scienceA porcine model to study the effect of brain death on kidney genomic responses.PMC6374499UL1 TR001414Hutchens MKhoshnevis MRadom-Aizik SSally MBZaldivar FPLe TPinette WAMalinoski DJfalseA porcine model to study the effect of brain death on kidney genomic responses.<h4>Introduction</h4>A majority of transplanted organs come from donors after brain death (BD). Renal grafts from these donors have higher delayed graft function and lower long-term survival rates compared to living donors. We designed a novel porcine BD model to better delineate the incompletely understood inflammatory response to BD, hypothesizing that adhesion molecule pathways would be upregulated in BD.<h4>Methods</h4>Animals were anesthetized and instrumented with monitors and a balloon catheter, then randomized to control and BD groups. BD was induced by inflating the balloon catheter and animals were maintained for 6 hours. RNA was extracted from kidneys, and gene expression pattern was determined.<h4>Results</h4>In total, 902 gene pairs were differently expressed between groups. E2018-01-01T00:00:00Z2018 Aug2026-05-03T15:39:26.763Z2019-06-06T20:52:02ZS-EPMC63744993080047810.1017/cts.2018.312