biostudies-literatureFlorentino APGoverno BrasilBrazilian GovernmentConselho Nacional de Desenvolvimento Científico e TecnológicoEuropean Research CouncilNetherlands Ministry of Education, Culture and ScienceMinistry of Education, Culture and Science, Netherlands209-225https://www.ebi.ac.uk/biostudies/studies/S-EPMC6378623biostudies-literatureUnknown21(1)Many questions regarding proteins involved in microbial sulfur metabolism remain unsolved. For sulfur respiration at low pH, the terminal electron acceptor is still unclear. Desulfurella amilsii is a sulfur-reducing bacterium that respires elemental sulfur (S⁰ ) or thiosulfate, and grows by S⁰ disproportionation. Due to its versatility, comparative studies on D. amilsii may shed light on microbial sulfur metabolism. Requirement of physical contact between cells and S⁰ was analyzed. Sulfide production decreased by around 50% when S⁰ was trapped in dialysis membranes, suggesting that contact between cells and S⁰ is beneficial, but not strictly needed. Proteome analysis was performed under the aforementioned conditions. A Mo-oxidoreductasEnvironmental microbiologyInsight into the sulfur metabolism of Desulfurella amilsii by differential proteomics.PMC6378623024.002.002323009Florentino APvan den Born MStams AJMSanchez-Andrea IPereira IACBoeren SfalseInsight into the sulfur metabolism of Desulfurella amilsii by differential proteomics.Many questions regarding proteins involved in microbial sulfur metabolism remain unsolved. For sulfur respiration at low pH, the terminal electron acceptor is still unclear. Desulfurella amilsii is a sulfur-reducing bacterium that respires elemental sulfur (S⁰ ) or thiosulfate, and grows by S⁰ disproportionation. Due to its versatility, comparative studies on D. amilsii may shed light on microbial sulfur metabolism. Requirement of physical contact between cells and S⁰ was analyzed. Sulfide production decreased by around 50% when S⁰ was trapped in dialysis membranes, suggesting that contact between cells and S⁰ is beneficial, but not strictly needed. Proteome analysis was performed under the aforementioned conditions. A Mo-oxidoreductas2019-01-01T00:00:00Z2019 Jan2026-05-01T10:24:59.764Z2019-06-06T20:52:38ZS-EPMC63786233030710410.1111/1462-2920.14442