{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hoffer ED"],"funding":["NIH-ORIP","Argonne National Laboratory","National Institutes of Health","National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["1557-1563"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6379664"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["47(3)"],"pubmed_abstract":["Decoding is thought to be governed by a conformational transition in the ribosome-open (off) to closed (on)-that occurs upon codon-anticodon pairing in the A site. Ribosomal ambiguity (ram) mutations increase miscoding and map to disparate regions, consistent with a role for ribosome dynamics in decoding, yet precisely how these mutations act has been unclear. Here, we solved crystal structures of 70S ribosomes harboring 16S ram mutations G299A and G347U in the absence A-site tRNA (A-tRNA) and in the presence of a near-cognate anticodon stem-loop (ASL). In the absence of an A-tRNA, each of the mutant ribosomes exhibits a partially closed (on) state. In the 70S-G347U structure, the 30S shoulder is rotated inward and intersubunit bridge B8 is disrupted. In the 70S-G299A structure, the 30S sh"],"journal":["Nucleic acids research"],"pubmed_title":["Ribosomal ambiguity (ram) mutations promote the open (off) to closed (on) transition and thereby increase miscoding."],"pmcid":["PMC6379664"],"funding_grant_id":["R01 GM093278","DE-AC02-06CH11357","GM093278","S10 RR029205","GM072528","R01 GM072528","P41 GM103403"],"pubmed_authors":["Hoffer ED","Dunham CM","Fredrick K","Maehigashi T"],"additional_accession":[]},"is_claimable":false,"name":"Ribosomal ambiguity (ram) mutations promote the open (off) to closed (on) transition and thereby increase miscoding.","description":"Decoding is thought to be governed by a conformational transition in the ribosome-open (off) to closed (on)-that occurs upon codon-anticodon pairing in the A site. Ribosomal ambiguity (ram) mutations increase miscoding and map to disparate regions, consistent with a role for ribosome dynamics in decoding, yet precisely how these mutations act has been unclear. Here, we solved crystal structures of 70S ribosomes harboring 16S ram mutations G299A and G347U in the absence A-site tRNA (A-tRNA) and in the presence of a near-cognate anticodon stem-loop (ASL). In the absence of an A-tRNA, each of the mutant ribosomes exhibits a partially closed (on) state. In the 70S-G347U structure, the 30S shoulder is rotated inward and intersubunit bridge B8 is disrupted. In the 70S-G299A structure, the 30S sh","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Feb","modification":"2026-05-04T16:30:27.351Z","creation":"2019-06-06T20:52:47Z"},"accession":"S-EPMC6379664","cross_references":{"pubmed":["30476222"],"doi":["10.1093/nar/gky1178"]}}