{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Song Y"],"funding":["NIA NIH HHS","NHLBI NIH HHS"],"pagination":["190016"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6380223"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["6"],"pubmed_abstract":["The immortalized human ReNcell VM cell line represents a reproducible and easy-to-propagate cell culture system for studying the differentiation of neural progenitors. To better characterize the starting line and its subsequent differentiation, we assessed protein and phospho-protein levels and cell morphology over a 15-day period during which ReNcell progenitors differentiated into neurons, astrocytes and oligodendrocytes. Five of the resulting datasets measured protein levels or states of phosphorylation based on tandem-mass-tag (TMT) mass spectrometry and four datasets characterized cellular phenotypes using high-content microscopy. Proteomic analysis revealed reproducible changes in pathways responsible for cytoskeletal rearrangement, cell phase transitions, neuronal migration, glial d"],"journal":["Scientific data"],"pubmed_title":["A dynamic view of the proteomic landscape during differentiation of ReNcell VM cells, an immortalized human neural progenitor line."],"pmcid":["PMC6380223"],"funding_grant_id":["R01 AG058063","R56 AG058063","U54 HL127365"],"pubmed_authors":["Sorger PK","Berberich MJ","Luria CM","Subramanian K","Rodriguez S","Everley R","Latorre IJ","Albers MW","Mitchison TJ","Song Y"],"additional_accession":[]},"is_claimable":false,"name":"A dynamic view of the proteomic landscape during differentiation of ReNcell VM cells, an immortalized human neural progenitor line.","description":"The immortalized human ReNcell VM cell line represents a reproducible and easy-to-propagate cell culture system for studying the differentiation of neural progenitors. To better characterize the starting line and its subsequent differentiation, we assessed protein and phospho-protein levels and cell morphology over a 15-day period during which ReNcell progenitors differentiated into neurons, astrocytes and oligodendrocytes. Five of the resulting datasets measured protein levels or states of phosphorylation based on tandem-mass-tag (TMT) mass spectrometry and four datasets characterized cellular phenotypes using high-content microscopy. Proteomic analysis revealed reproducible changes in pathways responsible for cytoskeletal rearrangement, cell phase transitions, neuronal migration, glial d","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Feb","modification":"2026-04-08T02:03:29.818Z","creation":"2019-06-06T20:52:53Z"},"accession":"S-EPMC6380223","cross_references":{"pubmed":["30778261"],"doi":["10.1038/sdata.2019.16"]}}