biostudies-literatureSong YNIA NIH HHSNHLBI NIH HHS190016https://www.ebi.ac.uk/biostudies/studies/S-EPMC6380223biostudies-literatureUnknown6The immortalized human ReNcell VM cell line represents a reproducible and easy-to-propagate cell culture system for studying the differentiation of neural progenitors. To better characterize the starting line and its subsequent differentiation, we assessed protein and phospho-protein levels and cell morphology over a 15-day period during which ReNcell progenitors differentiated into neurons, astrocytes and oligodendrocytes. Five of the resulting datasets measured protein levels or states of phosphorylation based on tandem-mass-tag (TMT) mass spectrometry and four datasets characterized cellular phenotypes using high-content microscopy. Proteomic analysis revealed reproducible changes in pathways responsible for cytoskeletal rearrangement, cell phase transitions, neuronal migration, glial dScientific dataA dynamic view of the proteomic landscape during differentiation of ReNcell VM cells, an immortalized human neural progenitor line.PMC6380223R01 AG058063R56 AG058063U54 HL127365Sorger PKBerberich MJLuria CMSubramanian KRodriguez SEverley RLatorre IJAlbers MWMitchison TJSong YfalseA dynamic view of the proteomic landscape during differentiation of ReNcell VM cells, an immortalized human neural progenitor line.The immortalized human ReNcell VM cell line represents a reproducible and easy-to-propagate cell culture system for studying the differentiation of neural progenitors. To better characterize the starting line and its subsequent differentiation, we assessed protein and phospho-protein levels and cell morphology over a 15-day period during which ReNcell progenitors differentiated into neurons, astrocytes and oligodendrocytes. Five of the resulting datasets measured protein levels or states of phosphorylation based on tandem-mass-tag (TMT) mass spectrometry and four datasets characterized cellular phenotypes using high-content microscopy. Proteomic analysis revealed reproducible changes in pathways responsible for cytoskeletal rearrangement, cell phase transitions, neuronal migration, glial d2019-01-01T00:00:00Z2019 Feb2026-04-08T02:03:29.818Z2019-06-06T20:52:53ZS-EPMC63802233077826110.1038/sdata.2019.16