{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["O hAinmhire E"],"funding":["NIDDK NIH HHS","Chan Zuckerberg Initiative","NIDDK"],"pagination":["F63-F75"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6383201"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["316(1)"],"pubmed_abstract":["Glioma-associated oncogene homolog-1 (Gli1)-positive resident mesenchymal stem cell-like cells are the predominant source of kidney myofibroblasts in fibrosis, but investigating Gli1-positive myofibroblast progenitor activation is hampered by the difficulty of isolating and propagating primary cultures of these cells. Using a genetic strategy with positive and negative selection, we isolated Kidney-Gli1 (KGli1) cells that maintain expression of appropriate mesenchymal stem cell-like cell markers, respond to hedgehog pathway activation, and display robust myofibroblast differentiation upon treatment with transforming growth factor-β (TGF-β). Coculture of KGli1 cells with endothelium stabilizes capillary formation. Single-cell RNA sequencing (scRNA-seq) analysis during differentiation identi"],"journal":["American journal of physiology. Renal physiology"],"pubmed_title":["A conditionally immortalized Gli1-positive kidney mesenchymal cell line models myofibroblast transition."],"pmcid":["PMC6383201"],"funding_grant_id":["DK076169","R01 DK103740","DK107374","UH3 DK107374","U24 DK115255","DK115255","173970","T32 DK007126","DK103740"],"pubmed_authors":["Muto Y","Donnelly EL","Humphreys BD","Machado FG","Fan LX","O hAinmhire E","Chang-Panesso M","Wu H"],"additional_accession":[]},"is_claimable":false,"name":"A conditionally immortalized Gli1-positive kidney mesenchymal cell line models myofibroblast transition.","description":"Glioma-associated oncogene homolog-1 (Gli1)-positive resident mesenchymal stem cell-like cells are the predominant source of kidney myofibroblasts in fibrosis, but investigating Gli1-positive myofibroblast progenitor activation is hampered by the difficulty of isolating and propagating primary cultures of these cells. Using a genetic strategy with positive and negative selection, we isolated Kidney-Gli1 (KGli1) cells that maintain expression of appropriate mesenchymal stem cell-like cell markers, respond to hedgehog pathway activation, and display robust myofibroblast differentiation upon treatment with transforming growth factor-β (TGF-β). Coculture of KGli1 cells with endothelium stabilizes capillary formation. Single-cell RNA sequencing (scRNA-seq) analysis during differentiation identi","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Jan","modification":"2026-07-10T03:23:05.218Z","creation":"2025-05-29T22:28:28.519Z"},"accession":"S-EPMC6383201","cross_references":{"pubmed":["30303712"],"doi":["10.1152/ajprenal.00460.2018"]}}