biostudies-literatureKirk EAAlexander Graham Bell ScholarshipNatural Sciences and Engineering Council of Canada Discovery Grant10-11https://www.ebi.ac.uk/biostudies/studies/S-EPMC6383552biostudies-literatureUnknown51(1)The ATP2A2 gene encodes the SERCA protein required for active calcium reuptake to the sarcoplasmic reticulum in cardiac and slow-twitch skeletal muscle. The ATP2A2 rs3026468 variant has been associated with voluntary strength phenotypes in humans but requires further validation. Here we investigated a homogenous cohort of 80 young, healthy, active Caucasian males who were assessed for maximal isometric strength, voluntary activation, stimulated contractile properties, and muscle potentiation in the quadriceps. A dynamometer was used to record knee extensions, and electrical stimulation was applied to the thigh to elicit a twitch response. DNA was isolated from cheek swabs, and the rs3026468 genotypes were assessed by TaqMan primer quantitative PCR. The results show no association between APhysiological genomicsATP2A2 rs3026468 does not associate with quadriceps contractile properties and acute muscle potentiation in humans.PMC6383552180970CGSD3 - 519372 - 2018Singh SMKirk EARice CLfalseATP2A2 rs3026468 does not associate with quadriceps contractile properties and acute muscle potentiation in humans.The ATP2A2 gene encodes the SERCA protein required for active calcium reuptake to the sarcoplasmic reticulum in cardiac and slow-twitch skeletal muscle. The ATP2A2 rs3026468 variant has been associated with voluntary strength phenotypes in humans but requires further validation. Here we investigated a homogenous cohort of 80 young, healthy, active Caucasian males who were assessed for maximal isometric strength, voluntary activation, stimulated contractile properties, and muscle potentiation in the quadriceps. A dynamometer was used to record knee extensions, and electrical stimulation was applied to the thigh to elicit a twitch response. DNA was isolated from cheek swabs, and the rs3026468 genotypes were assessed by TaqMan primer quantitative PCR. The results show no association between A2019-01-01T00:00:00Z2019 Jan2025-04-05T15:17:34.041Z2019-06-06T20:53:25ZS-EPMC63835523052631210.1152/physiolgenomics.00085.2018