biostudies-literatureIwahori KMEXT | Japan Society for the Promotion of Science2636https://www.ebi.ac.uk/biostudies/studies/S-EPMC6385254biostudies-literatureUnknown9(1)Cancer immunotherapy, including immune checkpoint inhibitors, exerts beneficial effects in cancer patients. However, immune checkpoint inhibitors are only advantageous for a limited population of cancer patients. Therefore, companion diagnostics are needed in order to identify patients for whom these therapies are effective. In the present study, we evaluated detailed immunological aspects in clinical specimens from non-small cell lung cancer (NSCLC) patients. We analyzed the immune profiles, T cell cytotoxicity, and TCR repertoire of peripheral blood, normal lung tissue, and tumor tissue from NSCLC patients. By using bispecific T-cell engager technology to assess the cytotoxicity of T cells, we found that the cytotoxicity of tumor-infiltrated T cells closely correlated with that of periphScientific reportsPeripheral T cell cytotoxicity predicts T cell function in the tumor microenvironment.PMC638525418K08143Morimoto-Okazawa ASato EOkumura MYoshida TIwahori KGottschalk SWada HYamamoto YShintani YFunaki SKawashima AMatsumoto MKumanogoh AfalsePeripheral T cell cytotoxicity predicts T cell function in the tumor microenvironment.Cancer immunotherapy, including immune checkpoint inhibitors, exerts beneficial effects in cancer patients. However, immune checkpoint inhibitors are only advantageous for a limited population of cancer patients. Therefore, companion diagnostics are needed in order to identify patients for whom these therapies are effective. In the present study, we evaluated detailed immunological aspects in clinical specimens from non-small cell lung cancer (NSCLC) patients. We analyzed the immune profiles, T cell cytotoxicity, and TCR repertoire of peripheral blood, normal lung tissue, and tumor tissue from NSCLC patients. By using bispecific T-cell engager technology to assess the cytotoxicity of T cells, we found that the cytotoxicity of tumor-infiltrated T cells closely correlated with that of periph2019-01-01T00:00:00Z2019 Feb2026-05-07T11:20:41.767Z2019-08-04T07:37:10ZS-EPMC63852543079631010.1038/s41598-019-39345-5