biostudies-literatureXu LNatural Science Foundation for Young Scientists and the Science & Technology Planning Project of Gansu ProvinceNatural Science Foundation for Young Scientists and the Science &amp; Technology Planning Project of Gansu ProvinceChinese foundation for hepatitis prevention and control——TianQing liver disease research fund subjectNational Natural Science Foundation of ChinaKey Technology Research and Development Program of the Sichuan ProvinceChinese foundation for hepatitis prevention and control--TianQing liver disease research fund subject98https://www.ebi.ac.uk/biostudies/studies/S-EPMC6385474biostudies-literatureUnknown38(1)<h4>Background</h4>Circular RNAs (circRNAs) play important roles in tumourigenesis and tumour progression. However, the expression profiles and functions of circRNAs in hepatocellular carcinoma (HCC) are largely unclear.<h4>Methods</h4>The expression profiles of circRNAs in HCC were identified through microarray analysis and were validated through quantitative reverse transcription polymerase chain reaction (qRT-PCR). Survival curves were plotted using the Kaplan-Meier method and compared using the log-rank test. The circular structure of candidate circRNA was confirmed through Sanger sequencing, divergent primer PCR, and RNase R treatments. Proliferation of HCC cells was evaluated in vitro and in vivo. The microRNA (miRNA) sponge mechanism of circRNAs was demonstrated using dual-luciferasJournal of experimental & clinical cancer research : CRCircSETD3 (Hsa_circ_0000567) acts as a sponge for microRNA-421 inhibiting hepatocellular carcinoma growth.PMC6385474TQGB2019020218JR3RA0582015SZ0131716731932017FZ008219ZDYF1740Xu LWang PZheng XXu MFeng XZhang YHao XZhang MLi LRen SfalseCircSETD3 (Hsa_circ_0000567) acts as a sponge for microRNA-421 inhibiting hepatocellular carcinoma growth.<h4>Background</h4>Circular RNAs (circRNAs) play important roles in tumourigenesis and tumour progression. However, the expression profiles and functions of circRNAs in hepatocellular carcinoma (HCC) are largely unclear.<h4>Methods</h4>The expression profiles of circRNAs in HCC were identified through microarray analysis and were validated through quantitative reverse transcription polymerase chain reaction (qRT-PCR). Survival curves were plotted using the Kaplan-Meier method and compared using the log-rank test. The circular structure of candidate circRNA was confirmed through Sanger sequencing, divergent primer PCR, and RNase R treatments. Proliferation of HCC cells was evaluated in vitro and in vivo. The microRNA (miRNA) sponge mechanism of circRNAs was demonstrated using dual-luciferas2019-01-01T00:00:00Z2019 Feb2026-07-16T19:14:32.852Z2019-08-03T07:21:13ZS-EPMC63854743079578710.1186/s13046-019-1041-2