biostudies-literatureWook Kim KUS National Institutes of HealthJuvenile Diabetes Research Foundation (JDRF)/NHMRC Centre of Research Excellence for the Protection of Pancreatic Beta CellsNIAID NIH HHSAustralian National Health and Medical Research Council (NHMRC) Practitioner fellowshipsofz025https://www.ebi.ac.uk/biostudies/studies/S-EPMC6386807biostudies-literatureUnknown6(2)<h4>Background</h4>The importance of gut bacteria in human physiology, immune regulation, and disease pathogenesis is well established. In contrast, the composition and dynamics of the gut virome are largely unknown; particularly lacking are studies in pregnancy. We used comprehensive virome capture sequencing to characterize the gut virome of pregnant women with and without type 1 diabetes (T1D), longitudinally followed in the Environmental Determinants of Islet Autoimmunity study.<h4>Methods</h4>In total, 61 pregnant women (35 with T1D and 26 without) from Australia were examined. Nucleic acid was extracted from serial fecal specimens obtained at prenatal visits, and viral genomes were sequenced by virome capture enrichment. The frequency, richness, and abundance of viruses were comparedOpen forum infectious diseasesDistinct Gut Virome Profile of Pregnant Women With Type 1 Diabetes in the ENDIA Study.PMC6386807APP1045777APP1044694APP1078106U19 AI109761Morahan GRawlinson WDWentworth JMGiles LCAllen DWPang ICNSinnott ROBriese TCraig MEDavis EAPenno MASHarris MThomson RLWook Kim KCotterill AMMorbey CLipkin WIPapenfuss ATBarry SCJain KCouper JJHarrison LCColman PGVuillermin PJIsaacs SRHorton JLHaynes ASoldatos GENDIA Study GroupWilkins MRfalseDistinct Gut Virome Profile of Pregnant Women With Type 1 Diabetes in the ENDIA Study.<h4>Background</h4>The importance of gut bacteria in human physiology, immune regulation, and disease pathogenesis is well established. In contrast, the composition and dynamics of the gut virome are largely unknown; particularly lacking are studies in pregnancy. We used comprehensive virome capture sequencing to characterize the gut virome of pregnant women with and without type 1 diabetes (T1D), longitudinally followed in the Environmental Determinants of Islet Autoimmunity study.<h4>Methods</h4>In total, 61 pregnant women (35 with T1D and 26 without) from Australia were examined. Nucleic acid was extracted from serial fecal specimens obtained at prenatal visits, and viral genomes were sequenced by virome capture enrichment. The frequency, richness, and abundance of viruses were compared2019-01-01T00:00:00Z2019 Feb2026-07-16T20:00:33.76Z2026-07-09T13:15:53.609ZS-EPMC63868073081550210.1093/ofid/ofz025