{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Montenegro Gouveia S"],"funding":["European Molecular Biology Organization","European Research Council","FP7 People: Marie-Curie Actions","Foundation for the National Institutes of Health","Funda??o para a Ci?ncia e a Tecnologia","Company of Biologists"],"pagination":["jcs219501"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6398482"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["132(4)"],"pubmed_abstract":["The centrosome is an important microtubule-organising centre (MTOC) in animal cells. It consists of two barrel-shaped structures, the centrioles, surrounded by the pericentriolar material (PCM), which nucleates microtubules. Centrosomes can form close to an existing structure (canonical duplication) or de novo How centrosomes form de novo is not known. The master driver of centrosome biogenesis, PLK4, is critical for the recruitment of several centriole components. Here, we investigate the beginning of centrosome biogenesis, taking advantage of Xenopus egg extracts, where PLK4 can induce de novo MTOC formation ( Eckerdt et al., 2011; Zitouni et al., 2016). Surprisingly, we observe that in vitro, PLK4 can self-assemble into condensates that recruit α- and β-tubulins. In Xenopus extracts, PL"],"journal":["Journal of cell science"],"pubmed_title":["PLK4 is a microtubule-associated protein that self-assembles promoting de novo MTOC formation."],"pmcid":["PMC6398482"],"funding_grant_id":["ALTF 1088-2009","683258","ERC-COG-683258","#253373"],"pubmed_authors":["Montenegro Gouveia S","Zitouni S","Hyman A","Tranfield EM","Loncarek J","Duarte P","Ferreira Gomes B","Sousa AL","Kong D","Bettencourt-Dias M"],"additional_accession":[]},"is_claimable":false,"name":"PLK4 is a microtubule-associated protein that self-assembles promoting de novo MTOC formation.","description":"The centrosome is an important microtubule-organising centre (MTOC) in animal cells. It consists of two barrel-shaped structures, the centrioles, surrounded by the pericentriolar material (PCM), which nucleates microtubules. Centrosomes can form close to an existing structure (canonical duplication) or de novo How centrosomes form de novo is not known. The master driver of centrosome biogenesis, PLK4, is critical for the recruitment of several centriole components. Here, we investigate the beginning of centrosome biogenesis, taking advantage of Xenopus egg extracts, where PLK4 can induce de novo MTOC formation ( Eckerdt et al., 2011; Zitouni et al., 2016). Surprisingly, we observe that in vitro, PLK4 can self-assemble into condensates that recruit α- and β-tubulins. In Xenopus extracts, PL","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Nov","modification":"2025-05-29T19:41:38.485Z","creation":"2025-05-29T19:41:38.485Z"},"accession":"S-EPMC6398482","cross_references":{"pubmed":["30237222"],"doi":["10.1242/jcs.219501"]}}