biostudies-literatureSnyder NANICHD NIH HHSNIAID NIH HHSNIGMS NIH HHS829-840https://www.ebi.ac.uk/biostudies/studies/S-EPMC6404609biostudies-literatureUnknown9(3)Gene knockout and knockdown strategies have been immensely successful probes of gene function, but small molecule inhibitors (SMIs) of gene products allow much greater time resolution and are particularly useful when the targets are essential for cell replication or survival. SMIs also serve as lead compounds for drug discovery. However, discovery of selective SMIs is costly and inefficient. The action of SMIs can be modeled simply by tagging gene products with an auxin-inducible degron (AID) that triggers rapid ubiquitylation and proteasomal degradation of the tagged protein upon exposure of live cells to auxin. To determine if this approach is broadly effective, we AID-tagged over 750 essential proteins in <i>Saccharomyces cerevisiae</i> and observed growth inhibition by low concentratioG3 (Bethesda, Md.)Auxin-Inducible Depletion of the Essentialome Suggests Inhibition of TORC1 by Auxins and Inhibition of Vrg4 by SDZ 90-215, a Natural Antifungal Cyclopeptide.PMC6404609T32 GM007231R21 HD080102R21 AI115016Roggo SKim AKester LGale ANSnyder NAHoepfner DStuder CHelliwell SBCunningham KWfalseAuxin-Inducible Depletion of the Essentialome Suggests Inhibition of TORC1 by Auxins and Inhibition of Vrg4 by SDZ 90-215, a Natural Antifungal Cyclopeptide.Gene knockout and knockdown strategies have been immensely successful probes of gene function, but small molecule inhibitors (SMIs) of gene products allow much greater time resolution and are particularly useful when the targets are essential for cell replication or survival. SMIs also serve as lead compounds for drug discovery. However, discovery of selective SMIs is costly and inefficient. The action of SMIs can be modeled simply by tagging gene products with an auxin-inducible degron (AID) that triggers rapid ubiquitylation and proteasomal degradation of the tagged protein upon exposure of live cells to auxin. To determine if this approach is broadly effective, we AID-tagged over 750 essential proteins in <i>Saccharomyces cerevisiae</i> and observed growth inhibition by low concentratio2019-01-01T00:00:00Z2019 Mar2025-04-18T14:18:37.696Z2019-06-06T20:57:47ZS-EPMC64046093067060810.1534/g3.118.200748