{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sobiak B"],"funding":["Narodowe Centrum Nauki"],"pagination":["E914"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6413078"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["20(4)"],"pubmed_abstract":["Epidermal differentiation is a complex process and its regulation may involve epigenetic factors. Analysis of DNA methylation in 20 selected regions within the epidermal differentiation complex (EDC) gene cluster by targeted next-generation sequencing (NGS) detected no or only minor changes in methylation, mostly slight demethylation, occurring during the course of keratinocyte differentiation. However, a single CpG pair within the exon of the <i>PGLYRP3</i> gene underwent a pronounced demethylation concomitant with an increase in <i>PGLYRP3</i> expression. We have employed a DNA-affinity precipitation assay (DAPA) and mass spectrometry to examine changes in the composition of proteins that bind to DNA containing either methylated or unmethylated CpG. We found that the unmethylated probe a"],"journal":["International journal of molecular sciences"],"pubmed_title":["The Effect of Single CpG Demethylation on the Pattern of DNA-Protein Binding."],"pmcid":["PMC6413078"],"funding_grant_id":["2016/23/N/NZ2/02376","2012/05/B/NZ2/02944"],"pubmed_authors":["Lesniak W","Sobiak B"],"additional_accession":[]},"is_claimable":false,"name":"The Effect of Single CpG Demethylation on the Pattern of DNA-Protein Binding.","description":"Epidermal differentiation is a complex process and its regulation may involve epigenetic factors. Analysis of DNA methylation in 20 selected regions within the epidermal differentiation complex (EDC) gene cluster by targeted next-generation sequencing (NGS) detected no or only minor changes in methylation, mostly slight demethylation, occurring during the course of keratinocyte differentiation. However, a single CpG pair within the exon of the <i>PGLYRP3</i> gene underwent a pronounced demethylation concomitant with an increase in <i>PGLYRP3</i> expression. We have employed a DNA-affinity precipitation assay (DAPA) and mass spectrometry to examine changes in the composition of proteins that bind to DNA containing either methylated or unmethylated CpG. We found that the unmethylated probe a","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Feb","modification":"2026-04-28T03:16:15.844Z","creation":"2019-06-06T20:59:11Z"},"accession":"S-EPMC6413078","cross_references":{"pubmed":["30791552"],"doi":["10.3390/ijms20040914"]}}