biostudies-literatureShapiguzov ADeutsche ForschungsgemeinschaftHorizon 2020 Framework ProgrammeHelsingin YliopistoFonds Wetenschappelijk OnderzoekSuomen Akatemiae43284https://www.ebi.ac.uk/biostudies/studies/S-EPMC6414205biostudies-literatureUnknown8Reactive oxygen species (ROS)-dependent signaling pathways from chloroplasts and mitochondria merge at the nuclear protein RADICAL-INDUCED CELL DEATH1 (RCD1). RCD1 interacts in vivo and suppresses the activity of the transcription factors ANAC013 and ANAC017, which mediate a ROS-related retrograde signal originating from mitochondrial complex III. Inactivation of <i>RCD1</i> leads to increased expression of mitochondrial dysfunction stimulon (MDS) genes regulated by ANAC013 and ANAC017. Accumulating MDS gene products, including alternative oxidases (AOXs), affect redox status of the chloroplasts, leading to changes in chloroplast ROS processing and increased protection of photosynthetic apparatus. ROS alter the abundance, thiol redox state and oligomerization of the RCD1 protein in vivo, peLifeArabidopsis RCD1 coordinates chloroplast and mitochondrial functions through interaction with ANAC transcription factors.PMC6414205Centre of Excellence Program 2014-1928823512D1815NDFG TRR 175Odysseus II G0D0515N312498Centre of Excellence Program 2006–11FPA No. 664620283139Centre of Excellence Program 2014–1927563225033630829584Centre of Excellence Program 2006-11Fernie ARKangasjarvi JTyystjarvi EPermi PDe Rybel BVan Breusegem FSipari NTiwari AHunter KAlseekh SKrasensky-Wrzaczek JBrosche MAarabi FVainonen JPRintamaki EAro EMJarvi SKeinanen MNikkanen LSalojarvi JWybouw BTossavainen HShapiguzov AHellman MVan Der Kelen KWrzaczek MfalseArabidopsis RCD1 coordinates chloroplast and mitochondrial functions through interaction with ANAC transcription factors.Reactive oxygen species (ROS)-dependent signaling pathways from chloroplasts and mitochondria merge at the nuclear protein RADICAL-INDUCED CELL DEATH1 (RCD1). RCD1 interacts in vivo and suppresses the activity of the transcription factors ANAC013 and ANAC017, which mediate a ROS-related retrograde signal originating from mitochondrial complex III. Inactivation of <i>RCD1</i> leads to increased expression of mitochondrial dysfunction stimulon (MDS) genes regulated by ANAC013 and ANAC017. Accumulating MDS gene products, including alternative oxidases (AOXs), affect redox status of the chloroplasts, leading to changes in chloroplast ROS processing and increased protection of photosynthetic apparatus. ROS alter the abundance, thiol redox state and oligomerization of the RCD1 protein in vivo, p2019-01-01T00:00:00Z2019 Feb2026-05-04T19:02:05.719Z2019-06-06T23:24:25ZS-EPMC64142053076789310.7554/eLife.43284