{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["9(1)"],"submitter":["Reheman A"],"pubmed_abstract":["We used Box-Behnken design-based (BBD) response surface methodology (RSM) in this research to optimize the extraction process of Traditional medicine Majun Mupakhi Ela (MME) and evaluate its effect on hydrocortisone-induced kidney yang deficiency. Three independent parameters were applied to evaluate the maximum phosphodiesterase type 5 (PDE5) inhibition activity of MME extracts in vitro. The optimal processing conditions (extraction time 2 h, solid-liquid ratio 1:16, extraction once) gave a maximum PDE5 inhibition rate of 84.10%, flavonoid content of 0.49 mg/ml, icariin content of 0.028 mg/ml and targeted extraction yield of 26.50%. In animal experiments, MME extracts significantly increased the adrenal mass index, semen weight index, preputial gland weight index, and penis weight index i"],"journal":["Scientific reports"],"pagination":["4628"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6420599"],"repository":["biostudies-literature"],"pubmed_title":["Optimization of Extraction Technology of Majun Mupakhi Ela and its Effect on Hydrocortisone-induced Kidney Yang Deficiency in Mice."],"pmcid":["PMC6420599"],"pubmed_authors":["Aisa HA","He F","Reheman A","Gao ZY","Wu T","Tursun X","Pu XP","Zhao X"],"additional_accession":[]},"is_claimable":false,"name":"Optimization of Extraction Technology of Majun Mupakhi Ela and its Effect on Hydrocortisone-induced Kidney Yang Deficiency in Mice.","description":"We used Box-Behnken design-based (BBD) response surface methodology (RSM) in this research to optimize the extraction process of Traditional medicine Majun Mupakhi Ela (MME) and evaluate its effect on hydrocortisone-induced kidney yang deficiency. Three independent parameters were applied to evaluate the maximum phosphodiesterase type 5 (PDE5) inhibition activity of MME extracts in vitro. The optimal processing conditions (extraction time 2 h, solid-liquid ratio 1:16, extraction once) gave a maximum PDE5 inhibition rate of 84.10%, flavonoid content of 0.49 mg/ml, icariin content of 0.028 mg/ml and targeted extraction yield of 26.50%. In animal experiments, MME extracts significantly increased the adrenal mass index, semen weight index, preputial gland weight index, and penis weight index i","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Mar","modification":"2026-07-17T00:45:52.032Z","creation":"2025-06-01T01:19:07.298Z"},"accession":"S-EPMC6420599","cross_references":{"pubmed":["30874604"],"doi":["10.1038/s41598-019-41006-6"]}}