biostudies-literatureVerheijen MEC | Seventh Framework Programme4641https://www.ebi.ac.uk/biostudies/studies/S-EPMC6420634biostudies-literatureUnknown9(1)Though clinical trials for medical applications of dimethyl sulfoxide (DMSO) reported toxicity in the 1960s, later, the FDA classified DMSO in the safest solvent category. DMSO became widely used in many biomedical fields and biological effects were overlooked. Meanwhile, biomedical science has evolved towards sensitive high-throughput techniques and new research areas, including epigenomics and microRNAs. Considering its wide use, especially for cryopreservation and in vitro assays, we evaluated biological effect of DMSO using these technological innovations. We exposed 3D cardiac and hepatic microtissues to medium with or without 0.1% DMSO and analyzed the transcriptome, proteome and DNA methylation profiles. In both tissue types, transcriptome analysis detected >2000 differentially exprScientific reportsDMSO induces drastic changes in human cellular processes and epigenetic landscape in vitro.PMC6420634602156Herwig RCaiment FGeraedts JGmuender HSchrooders YNudischer RGotta SSchlapbach RClayton OLienhard MBoerno SKleinjans JVerheijen MTimmermann BSelevsek NfalseDMSO induces drastic changes in human cellular processes and epigenetic landscape in vitro.Though clinical trials for medical applications of dimethyl sulfoxide (DMSO) reported toxicity in the 1960s, later, the FDA classified DMSO in the safest solvent category. DMSO became widely used in many biomedical fields and biological effects were overlooked. Meanwhile, biomedical science has evolved towards sensitive high-throughput techniques and new research areas, including epigenomics and microRNAs. Considering its wide use, especially for cryopreservation and in vitro assays, we evaluated biological effect of DMSO using these technological innovations. We exposed 3D cardiac and hepatic microtissues to medium with or without 0.1% DMSO and analyzed the transcriptome, proteome and DNA methylation profiles. In both tissue types, transcriptome analysis detected >2000 differentially expr2019-01-01T00:00:00Z2019 Mar2026-05-03T19:01:37.111Z2019-06-06T21:00:12ZS-EPMC64206343087458610.1038/s41598-019-40660-0