biostudies-literatureUnknown10Kostaki EGBackground and Aim: The circulating recombinant form 02_AG (CRF02_AG) is the predominant clade among the human immunodeficiency virus type-1 (HIV-1) non-Bs with a prevalence of 5.97% (95% Confidence Interval-CI: 5.41-6.57%) across Spain. Our aim was to estimate the levels of regional clustering for CRF02_AG and the spatiotemporal characteristics of the largest CRF02_AG subepidemic in Spain. Methods: We studied 396 CRF02_AG sequences obtained from HIV-1 diagnosed patients during 2000-2014 from 10 autonomous communities of Spain. Phylogenetic analysis was performed on the 391 CRF02_AG sequences along with all globally sampled CRF02_AG sequences (N = 3,302) as references. Phylodynamic and phylogeographic analysis was performed to the largest CRF02_AG monophyletic cluster by a Bayesian method Frontiers in microbiology370https://www.ebi.ac.uk/biostudies/studies/S-EPMC6421502biostudies-literatureSpatiotemporal Characteristics of the Largest HIV-1 CRF02_AG Outbreak in Spain: Evidence for Onward Transmissions.PMC6421502Giner LSerrano SMontero Alonso MMerino EGonzalez-Ruano PDronda FOlalla JGutierrez IAlcaraz Garcia AJimeno ARull ACastro Hernandez IMayoral MFerrer ERodriguez-Gallego EAntela AAldamiz-Echevarria TArrizabalaga JAlcaraz BGutierrez CGutierrez FRamirez MSanz Sanz JMiguel Garcia Sousa LSanz Moreno JJose Aramburu MJose Parras FViciana PAyerdi OGonzalez JSantacreu MVeloso SBlanes Julia MSilva ARiera MGomez Ayerbe CViciana IMoreno CPeraire JKaramitros TBernal ERubio RSantos JMoreno AMasia MSanchez-Paya JArnalich FIborra AIniesta CMontolio FGuerrero-Beltran CGarcia-Fraile Fraile LAmador CJesus Bustinduy MHontanon VGonzalez-Baeza APerez Stachowski JJose Alcaraz Mde Los Santos ILosada EDel Carmen Villalba MMurillas JHernando ACarlos Lopez Bernaldo de Quiros JMoreno SDominguez LRamon Arribas JCamino XMoreno VPerez-Martinez LGadea CLuis Blanco JA Pineda JAlejos BMaria Garcia de Lomas Guerrero JSanmarti MPulido FAlba VBravo Urbieta JAngustias Merlos MGracia Ruiz de Alda MCairo MHernandez JDel Arco AMalmierca EBalsalobre PRial Crestelo DAntonio Iribarren JGarcia-Bujalance SGarcia JDalmau DLizardi AGarcia EMartinez LGarcia FChueca NGarcia DAlberto Garcia JPascual RGarcia Lopez-Hortelano MVelli PGomez Rico CMetola LLopez-Dupla MPadilla SVidal CMontero VVidal ALeyes MPerez ALopez Aldeguer JMunoz LPadilla BGallego de la Fuente JBenito SMunoz ABallesteros JNavarro AMarti PAlvarez MArranz Caso AGijon PReparaz JSanz Perez NRemedios Aleman MPena AAngel Goenaga MSendagorta EPenaranda MTeresa de Leon Cano MCano ALuisa Navarro MSaumoy MKortajarena XJesus Vivancos MAlonso NSuarez-Garcia ICoRISDel Campo Terron SMaria Garcia-Merino ISalas Aparicio ARamon Blanco JDelgado ESainz TCarlos Rodriguez JFont RFernandez Navarro JMacias JGonzalez-Garcia JVidal FLuz Martin-Carbonero MValencia ECarlos Carrio JJarrin IIbarguren MMaria Gonzalez CBermejo LAzkune HDel Mar Alonso MFlampouris AThomson MConsuegra Fernandez IIsabel Gonzalez MPalacios RSanz MKostaki EGBerenguer JMartinez-Montes CLuis Gomez JHernandez Gutierrez CHerranz PMontejano RRodriguez-Arrondo FTasias Pitarch MVargas MDel Romero JAntonio Oteo JMena de Cea ACasas PFernandez MJesus Vera FJuan Martinez OHomar FPortilla JPortilla IParaskevis DVera MXercavins MLuis Casado JAngels Ribas MPernas Souto BAngel von Wichmann MCalabuig Munoz EBoix VDiaz-Flores FAdsuar ASarria Cepeda CPodzamczer DPalau Concejo PIgnacio Bernardino de la Serna JVinuesa DPierola Ruiz de Galarreta BPelazas RBarber XSalavert Lleti MPerez Latorre LOlona MIbarra VMartin Rodrigo DReus SHernando VVilades CPascual Tome LPuerta TLuisa Montes MLuis Prada JOmar Mohamed-Balghata MEsteban Mican RCastro Iglesias AJaen ARodes BInmaculada Hernandez MRobledano CMartinez-Lacasa JJose Mellado MGalera CMiguel Molina JDiez CFanjul FEspinosa NDiez MRodriguez CJesus Perez Elias MMiguel Castro JMadrid NBisbal ONovella Mena MFernando Lopez-Cortes LImaz APampliega MHatzakis AAlbendin HLopez Lirola AStella Alcariz NPerez-Valero IEscosa LTiraboschi JGregori Colome JTejerina FMartin Gayo Ede la Torre JA Campins AAguilera AAngeles Munoz-Fernandez MCuellar Tovar SAnsa UfalseSpatiotemporal Characteristics of the Largest HIV-1 CRF02_AG Outbreak in Spain: Evidence for Onward Transmissions.Background and Aim: The circulating recombinant form 02_AG (CRF02_AG) is the predominant clade among the human immunodeficiency virus type-1 (HIV-1) non-Bs with a prevalence of 5.97% (95% Confidence Interval-CI: 5.41-6.57%) across Spain. Our aim was to estimate the levels of regional clustering for CRF02_AG and the spatiotemporal characteristics of the largest CRF02_AG subepidemic in Spain. Methods: We studied 396 CRF02_AG sequences obtained from HIV-1 diagnosed patients during 2000-2014 from 10 autonomous communities of Spain. Phylogenetic analysis was performed on the 391 CRF02_AG sequences along with all globally sampled CRF02_AG sequences (N = 3,302) as references. Phylodynamic and phylogeographic analysis was performed to the largest CRF02_AG monophyletic cluster by a Bayesian method 2019-01-01T00:00:00Z20192025-04-26T18:21:03.304Z2019-06-06T21:00:22ZS-EPMC64215023091504010.3389/fmicb.2019.00370