biostudies-literatureZhu XXin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, ChinaScience and Technology Commission of Shanghai Municipality, ChinaShanghai Municipal Commission of Health and Family Planning, China497-504https://www.ebi.ac.uk/biostudies/studies/S-EPMC6422475biostudies-literatureUnknown20(4)<h4>Objective</h4>Angiogenesis is one of the key processes in the development of malignant tumors. The vascular endothelial growth factor (VEGF) and VEGF receptor-2 (VEGFR-2) signaling pathway regulates branching angiogenesis in cancer. In this study, we analyzed the associations of VEGF/VEGFR-2 proteins and VEGFR-2 genetic variations with the prognosis of gastric cancer (GC).<h4>Method</h4>We collected the clinical information of patients with GC and extracted genomic DNA from paraffin-embedded tissues. Immunohistochemical methods were used to detect the expression of VEGF and VEGFR-2 in GC tissues. Four single nucleotide polymorphisms of VEGFR-2 were detected by the TaqMan assay. The Kaplan-Meier method and Cox regression model were applied to analyze the associations between clinicopathCancer biology & therapyThe VEGFR-2 protein and the VEGFR-2 rs1870377 A>T genetic polymorphism are prognostic factors for gastric cancer.PMC6422475Grant No.15ZR1427700Grant No.15LC05 and No. 13YJ22Grant No. 201540271Xue WWang RZheng LZhu XZhu MLWang YWang LfalseThe VEGFR-2 protein and the VEGFR-2 rs1870377 A>T genetic polymorphism are prognostic factors for gastric cancer.<h4>Objective</h4>Angiogenesis is one of the key processes in the development of malignant tumors. The vascular endothelial growth factor (VEGF) and VEGF receptor-2 (VEGFR-2) signaling pathway regulates branching angiogenesis in cancer. In this study, we analyzed the associations of VEGF/VEGFR-2 proteins and VEGFR-2 genetic variations with the prognosis of gastric cancer (GC).<h4>Method</h4>We collected the clinical information of patients with GC and extracted genomic DNA from paraffin-embedded tissues. Immunohistochemical methods were used to detect the expression of VEGF and VEGFR-2 in GC tissues. Four single nucleotide polymorphisms of VEGFR-2 were detected by the TaqMan assay. The Kaplan-Meier method and Cox regression model were applied to analyze the associations between clinicopath2019-01-01T00:00:00Z20192026-04-08T03:16:55.844Z2019-11-07T08:03:20ZS-EPMC64224753038097010.1080/15384047.2018.1537575