{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ji N"],"funding":["National Natural Science Foundation of China"],"pagination":["400"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6422896"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10"],"pubmed_abstract":["AlkB monooxygenases in bacteria are responsible for the hydroxylation of medium- and long-chain <i>n</i>-alkanes. In this study, one CrgA protein of <i>Pseudomonas aeruginosa</i> SJTD-1, a member of LysR family, was proved to regulate AlkB2 monooxygenase and the degradation of medium-to-long-chain <i>n</i>-alkanes (C<sub>14</sub>-C<sub>20</sub>) by directly binding to the upstream of <i>alkB</i>2 gene. Two specific sites for CrgA binding were found in the promoter region of <i>alkB</i>2 gene, and the imperfect mirror repeat (IIR) structure was proved critical for CrgA recognition and binding. Hexadecyl CoA and octadecyl CoA could effectively release the CrgA binding and start the transcription of <i>alkB2</i> gene, implying a positive regulation of metabolic intermediate. In the presence o"],"journal":["Frontiers in microbiology"],"pubmed_title":["CrgA Protein Represses AlkB2 Monooxygenase and Regulates the Degradation of Medium-to-Long-Chain <i>n</i>-Alkanes in <i>Pseudomonas aeruginosa</i> SJTD-1."],"pmcid":["PMC6422896"],"funding_grant_id":["31370152","31570099"],"pubmed_authors":["Peng W","Yin C","Wang X","Ji N","Liang R"],"additional_accession":[]},"is_claimable":false,"name":"CrgA Protein Represses AlkB2 Monooxygenase and Regulates the Degradation of Medium-to-Long-Chain <i>n</i>-Alkanes in <i>Pseudomonas aeruginosa</i> SJTD-1.","description":"AlkB monooxygenases in bacteria are responsible for the hydroxylation of medium- and long-chain <i>n</i>-alkanes. In this study, one CrgA protein of <i>Pseudomonas aeruginosa</i> SJTD-1, a member of LysR family, was proved to regulate AlkB2 monooxygenase and the degradation of medium-to-long-chain <i>n</i>-alkanes (C<sub>14</sub>-C<sub>20</sub>) by directly binding to the upstream of <i>alkB</i>2 gene. Two specific sites for CrgA binding were found in the promoter region of <i>alkB</i>2 gene, and the imperfect mirror repeat (IIR) structure was proved critical for CrgA recognition and binding. Hexadecyl CoA and octadecyl CoA could effectively release the CrgA binding and start the transcription of <i>alkB2</i> gene, implying a positive regulation of metabolic intermediate. In the presence o","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019","modification":"2026-04-07T14:37:01.299Z","creation":"2025-05-29T20:21:57.13Z"},"accession":"S-EPMC6422896","cross_references":{"pubmed":["30915046"],"doi":["10.3389/fmicb.2019.00400"]}}