biostudies-literatureTian XNCI NIH HHS8https://www.ebi.ac.uk/biostudies/studies/S-EPMC6423228biostudies-literatureUnknown3The development of genetic engineering in the 1970s marked a new frontier in genome-editing technology. Gene-editing technologies have provided a plethora of benefits to the life sciences. The clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9 (CRISPR/ Cas9) system is a versatile technology that provides the ability to add or remove DNA in the genome in a sequence-specific manner. Serious efforts are underway to improve the efficiency of CRISPR/Cas9 targeting and thus reduce off-target effects. Currently, various applications of CRISPR/Cas9 are used in cancer biology and oncology to perform robust site-specific gene editing, thereby becoming more useful for biological and clinical applications. Many variants and applications of CRISPR/Cas9 are being rapidNPJ precision oncologyCRISPR/Cas9 - An evolving biological tool kit for cancer biology and oncology.PMC6423228R01 CA166011R01 CA187027R01 CA196639Gu TLee MHTian XPatel SDong ZBode AMfalseCRISPR/Cas9 - An evolving biological tool kit for cancer biology and oncology.The development of genetic engineering in the 1970s marked a new frontier in genome-editing technology. Gene-editing technologies have provided a plethora of benefits to the life sciences. The clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9 (CRISPR/ Cas9) system is a versatile technology that provides the ability to add or remove DNA in the genome in a sequence-specific manner. Serious efforts are underway to improve the efficiency of CRISPR/Cas9 targeting and thus reduce off-target effects. Currently, various applications of CRISPR/Cas9 are used in cancer biology and oncology to perform robust site-specific gene editing, thereby becoming more useful for biological and clinical applications. Many variants and applications of CRISPR/Cas9 are being rapid2019-01-01T00:00:00Z20192025-05-29T20:27:18.943Z2025-05-29T20:27:18.943ZS-EPMC64232283091167610.1038/s41698-019-0080-7