{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Reis FCG"],"funding":["MCTI | Conselho Nacional de Desenvolvimento Científico e Tecnológico","Fundação Oswaldo Cruz"],"pagination":["e00080-19"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6429041"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["4(2)"],"pubmed_abstract":["Regular protocols for the isolation of fungal extracellular vesicles (EVs) are time-consuming, hard to reproduce, and produce low yields. In an attempt to improve the protocols used for EV isolation, we explored a model of vesicle production after growth of <i>Cryptococcus gattii</i> and <i>Cryptococcus neoformans</i> on solid media. Nanoparticle tracking analysis in combination with transmission electron microscopy revealed that <i>C. gattii</i> and <i>C. neoformans</i> produced EVs in solid media. The properties of cryptococcal vesicles varied according to the culture medium used and the EV-producing species. EV detection was reproduced with an acapsular mutant of <i>C. neoformans</i>, as well as with isolates of <i>Candida albicans</i>, <i>Histoplasma capsulatum</i>, and <i>Saccharomyce"],"journal":["mSphere"],"pubmed_title":["A Novel Protocol for the Isolation of Fungal Extracellular Vesicles Reveals the Participation of a Putative Scramblase in Polysaccharide Export and Capsule Construction in <i>Cryptococcus gattii</i>."],"pmcid":["PMC6429041"],"funding_grant_id":["408711/2017-7","301304/2017-3","VPPIS-001-FIO-18-66","311179/2017-7","405520/2018-2","440015/2018-9","VPPCB-007-FIO-18-2-57"],"pubmed_authors":["Garcia AWA","Honorato L","Alves LR","Reis FCG","Martins ST","Nimrichter L","Rodrigues ML","Vainstein MH","Medeiros LC","Staats CC","Borges BS","Kmetzsch L","Schrank A","Jozefowicz LJ","Sena BAG"],"additional_accession":[]},"is_claimable":false,"name":"A Novel Protocol for the Isolation of Fungal Extracellular Vesicles Reveals the Participation of a Putative Scramblase in Polysaccharide Export and Capsule Construction in <i>Cryptococcus gattii</i>.","description":"Regular protocols for the isolation of fungal extracellular vesicles (EVs) are time-consuming, hard to reproduce, and produce low yields. In an attempt to improve the protocols used for EV isolation, we explored a model of vesicle production after growth of <i>Cryptococcus gattii</i> and <i>Cryptococcus neoformans</i> on solid media. Nanoparticle tracking analysis in combination with transmission electron microscopy revealed that <i>C. gattii</i> and <i>C. neoformans</i> produced EVs in solid media. The properties of cryptococcal vesicles varied according to the culture medium used and the EV-producing species. EV detection was reproduced with an acapsular mutant of <i>C. neoformans</i>, as well as with isolates of <i>Candida albicans</i>, <i>Histoplasma capsulatum</i>, and <i>Saccharomyce","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Mar","modification":"2026-07-12T03:22:49.697Z","creation":"2026-07-12T03:13:02.452Z"},"accession":"S-EPMC6429041","cross_references":{"pubmed":["30894430"],"doi":["10.1128/mSphere.00080-19"]}}