{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Xiao Y"],"funding":["National High-end Foreign Expert Project of China","Major Projects of China National Science and Technology"],"pagination":["186-195"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6437641"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["43(2)"],"pubmed_abstract":["<h4>Background</h4>Notoginseng stem-leaf (NGL) ginsenosides have not been well used. To improve their utilization, the biotransformation of NGL ginsenosides was studied using ginsenosidase type-I from <i>Aspergillus niger</i> g.848.<h4>Methods</h4>NGL ginsenosides were reacted with a crude enzyme in the RAT-5D bioreactor, and the dynamic changes of multi-ginsenosides of NGL were recognized by HPLC. The reaction products were separated using a silica gel column and identified by HPLC and NMR.<h4>Results</h4>All the NGL ginsenosides are protopanaxadiol-type ginsenosides; the main ginsenoside contents are 27.1% Rb3, 15.7% C-Mx1, 13.8% Rc, 11.1% Fc, 7.10% Fa, 6.44% C-Mc, 5.08% Rb2, and 4.31% Rb1. In the reaction of NGL ginsenosides with crude enzyme, the main reaction of Rb3 and C-Mx1 occurred"],"journal":["Journal of ginseng research"],"pubmed_title":["Dynamic changes of multi-notoginseng stem-leaf ginsenosides in reaction with ginsenosidase type-I."],"pmcid":["PMC6437641"],"funding_grant_id":["GDT20152100019","2012ZX09503001-003"],"pubmed_authors":["Xu L","Jin F","Yi TH","Zuo K","Xiao Y","Liu C","Chen S","Yu H","Song J","Im WT"],"additional_accession":[]},"is_claimable":false,"name":"Dynamic changes of multi-notoginseng stem-leaf ginsenosides in reaction with ginsenosidase type-I.","description":"<h4>Background</h4>Notoginseng stem-leaf (NGL) ginsenosides have not been well used. To improve their utilization, the biotransformation of NGL ginsenosides was studied using ginsenosidase type-I from <i>Aspergillus niger</i> g.848.<h4>Methods</h4>NGL ginsenosides were reacted with a crude enzyme in the RAT-5D bioreactor, and the dynamic changes of multi-ginsenosides of NGL were recognized by HPLC. The reaction products were separated using a silica gel column and identified by HPLC and NMR.<h4>Results</h4>All the NGL ginsenosides are protopanaxadiol-type ginsenosides; the main ginsenoside contents are 27.1% Rb3, 15.7% C-Mx1, 13.8% Rc, 11.1% Fc, 7.10% Fa, 6.44% C-Mc, 5.08% Rb2, and 4.31% Rb1. In the reaction of NGL ginsenosides with crude enzyme, the main reaction of Rb3 and C-Mx1 occurred","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Apr","modification":"2025-04-18T20:26:24.431Z","creation":"2019-06-06T21:03:29Z"},"accession":"S-EPMC6437641","cross_references":{"pubmed":["30976159"],"doi":["10.1016/j.jgr.2017.10.001"]}}