biostudies-literatureXiao YNational High-end Foreign Expert Project of ChinaMajor Projects of China National Science and Technology186-195https://www.ebi.ac.uk/biostudies/studies/S-EPMC6437641biostudies-literatureUnknown43(2)<h4>Background</h4>Notoginseng stem-leaf (NGL) ginsenosides have not been well used. To improve their utilization, the biotransformation of NGL ginsenosides was studied using ginsenosidase type-I from <i>Aspergillus niger</i> g.848.<h4>Methods</h4>NGL ginsenosides were reacted with a crude enzyme in the RAT-5D bioreactor, and the dynamic changes of multi-ginsenosides of NGL were recognized by HPLC. The reaction products were separated using a silica gel column and identified by HPLC and NMR.<h4>Results</h4>All the NGL ginsenosides are protopanaxadiol-type ginsenosides; the main ginsenoside contents are 27.1% Rb3, 15.7% C-Mx1, 13.8% Rc, 11.1% Fc, 7.10% Fa, 6.44% C-Mc, 5.08% Rb2, and 4.31% Rb1. In the reaction of NGL ginsenosides with crude enzyme, the main reaction of Rb3 and C-Mx1 occurredJournal of ginseng researchDynamic changes of multi-notoginseng stem-leaf ginsenosides in reaction with ginsenosidase type-I.PMC6437641GDT201521000192012ZX09503001-003Xu LJin FYi THZuo KXiao YLiu CChen SYu HSong JIm WTfalseDynamic changes of multi-notoginseng stem-leaf ginsenosides in reaction with ginsenosidase type-I.<h4>Background</h4>Notoginseng stem-leaf (NGL) ginsenosides have not been well used. To improve their utilization, the biotransformation of NGL ginsenosides was studied using ginsenosidase type-I from <i>Aspergillus niger</i> g.848.<h4>Methods</h4>NGL ginsenosides were reacted with a crude enzyme in the RAT-5D bioreactor, and the dynamic changes of multi-ginsenosides of NGL were recognized by HPLC. The reaction products were separated using a silica gel column and identified by HPLC and NMR.<h4>Results</h4>All the NGL ginsenosides are protopanaxadiol-type ginsenosides; the main ginsenoside contents are 27.1% Rb3, 15.7% C-Mx1, 13.8% Rc, 11.1% Fc, 7.10% Fa, 6.44% C-Mc, 5.08% Rb2, and 4.31% Rb1. In the reaction of NGL ginsenosides with crude enzyme, the main reaction of Rb3 and C-Mx1 occurred2019-01-01T00:00:00Z2019 Apr2025-04-18T20:26:24.431Z2019-06-06T21:03:29ZS-EPMC64376413097615910.1016/j.jgr.2017.10.001