biostudies-literatureBouley JNICHD NIH HHSNCATS NIH HHSFDA HHSNINDS NIH HHS1008-1017https://www.ebi.ac.uk/biostudies/studies/S-EPMC6444888biostudies-literatureUnknown36(7)Cortical spreading depression (CSD) has been described after moderate-to-severe traumatic brain injury (TBI). It is uncertain, however, whether CSD occurs after mild, concussive TBI and whether it relates to brain pathology and functional outcome. Male C57BL6/J mice (n = 62) were subjected to closed head TBI with a 25 g weight (n = 11), 50 g weight (n = 45), or sham injury (n = 6). Laser Doppler flowmetry and optical intrinsic signal imaging were used to determine cerebral blood flow dynamics after concussive CSD. Functional deficits were assessed at baseline, 2 h, 24 h, and 48 h. TUNEL and Prussian blue staining were used to determine cell death and presence of cerebral microbleeds at 48 h. No CSD was observed in mice subjected to a 25 g weight drop whereas 58.9% of mice subjected to a 50Journal of neurotraumaCortical Spreading Depression Denotes Concussion Injury.PMC6444888K08 NS091499KL2 TR002542R01 NS102969R01 NS088689P50 HD060848P01 NS055104UL1 TR002541R25 NS065743U54 HD060848R01 FD004127Henninger NAyata CChung DYBouley JBrown RHfalseCortical Spreading Depression Denotes Concussion Injury.Cortical spreading depression (CSD) has been described after moderate-to-severe traumatic brain injury (TBI). It is uncertain, however, whether CSD occurs after mild, concussive TBI and whether it relates to brain pathology and functional outcome. Male C57BL6/J mice (n = 62) were subjected to closed head TBI with a 25 g weight (n = 11), 50 g weight (n = 45), or sham injury (n = 6). Laser Doppler flowmetry and optical intrinsic signal imaging were used to determine cerebral blood flow dynamics after concussive CSD. Functional deficits were assessed at baseline, 2 h, 24 h, and 48 h. TUNEL and Prussian blue staining were used to determine cell death and presence of cerebral microbleeds at 48 h. No CSD was observed in mice subjected to a 25 g weight drop whereas 58.9% of mice subjected to a 502019-01-01T00:00:00Z2019 Apr2025-04-26T14:04:12.625Z2025-02-18T22:56:57.059ZS-EPMC64448882999945510.1089/neu.2018.5844