biostudies-literatureBrun PUniversità degli Studi di Padova5583https://www.ebi.ac.uk/biostudies/studies/S-EPMC6447567biostudies-literatureUnknown9(1)Damage of enteric neurons and partial or total loss of selective neuronal populations are reported in intestinal disorders including inflammatory bowel diseases and necrotizing enterocolitis. To develop three-dimensional scaffolds for enteric neurons we propose the decoration of ionic-complementary self-assembling peptide (SAP) hydrogels, namely EAK or EAbuK, with bioactive motives. Our results showed the ability of EAK in supporting neuronal cell attachment and neurite development. Therefore, EAK was covalently conjugated to: RGD, (GRGDSP)₄K (fibronectin), FRHRNRKGY (h-vitronectin, named HVP), IKVAV (laminin), and type 1 Insulin-like Growth Factor (IGF-1). Chemoselective ligation was applied for the SAP conjugation with IGF-1 and the other longer sequences. Freshly isolated murScientific reports3D Synthetic Peptide-based Architectures for the Engineering of the Enteric Nervous System.PMC6447567PRAT2012DETT_SID17_01Peretti AZamuner ABrun PMessina GMLDettin MConti JMarletta Gfalse3D Synthetic Peptide-based Architectures for the Engineering of the Enteric Nervous System.Damage of enteric neurons and partial or total loss of selective neuronal populations are reported in intestinal disorders including inflammatory bowel diseases and necrotizing enterocolitis. To develop three-dimensional scaffolds for enteric neurons we propose the decoration of ionic-complementary self-assembling peptide (SAP) hydrogels, namely EAK or EAbuK, with bioactive motives. Our results showed the ability of EAK in supporting neuronal cell attachment and neurite development. Therefore, EAK was covalently conjugated to: RGD, (GRGDSP)₄K (fibronectin), FRHRNRKGY (h-vitronectin, named HVP), IKVAV (laminin), and type 1 Insulin-like Growth Factor (IGF-1). Chemoselective ligation was applied for the SAP conjugation with IGF-1 and the other longer sequences. Freshly isolated mur2019-01-01T00:00:00Z2019 Apr2026-05-03T19:18:17.148Z2019-06-05T16:16:09ZS-EPMC64475673094441010.1038/s41598-019-42071-7