biostudies-literatureLien PTKJapan Society for the Promotion of Sciencee0215064https://www.ebi.ac.uk/biostudies/studies/S-EPMC6459547biostudies-literatureUnknown14(4)The S. cerevisiae Pop2 protein is an exonuclease in the Ccr4-Not complex that is a conserved regulator of gene expression. Pop2 regulates gene expression post-transcriptionally by shortening the poly(A) tail of mRNA. A previous study has shown that Pop2 is phosphorylated at threonine 97 (T97) by Yak1 protein kinase in response to glucose limitation. However, the physiological importance of Pop2 phosphorylation remains unknown. In this study, we found that Pop2 is phosphorylated at serine 39 (S39) under unstressed conditions. The dephosphorylation of S39 was occurred rapidly after glucose depletion, and the addition of glucose to the glucose-deprived culture recovered this phosphorylation, suggesting that Pop2 phosphorylation at S39 is regulated by glucose. This glucose-regulated phosphorylPloS onePop2 phosphorylation at S39 contributes to the glucose repression of stress response genes, HSP12 and HSP26.PMC645954715K0694418K06053Mizuno TLien PTKViet NTMSuda YIrie KfalsePop2 phosphorylation at S39 contributes to the glucose repression of stress response genes, HSP12 and HSP26.The S. cerevisiae Pop2 protein is an exonuclease in the Ccr4-Not complex that is a conserved regulator of gene expression. Pop2 regulates gene expression post-transcriptionally by shortening the poly(A) tail of mRNA. A previous study has shown that Pop2 is phosphorylated at threonine 97 (T97) by Yak1 protein kinase in response to glucose limitation. However, the physiological importance of Pop2 phosphorylation remains unknown. In this study, we found that Pop2 is phosphorylated at serine 39 (S39) under unstressed conditions. The dephosphorylation of S39 was occurred rapidly after glucose depletion, and the addition of glucose to the glucose-deprived culture recovered this phosphorylation, suggesting that Pop2 phosphorylation at S39 is regulated by glucose. This glucose-regulated phosphoryl2019-01-01T00:00:00Z20192026-05-02T02:58:25.286Z2025-05-31T23:17:13.074ZS-EPMC64595473097394510.1371/journal.pone.0215064