{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["8(4)"],"submitter":["Dooley-Cullinane TM"],"pubmed_abstract":["Nitrilase enzymes (EC 3.5.5.1) are responsible for the direct hydration of nitriles to their corresponding carboxylic acids and ammonia. The utilization of nitrilase enzymes in biocatalysis toward bio-pharmaceuticals and industrial applications facilitates the move towards green chemistry. The body of research presented describes a novel clade-specific touchdown PCR protocol for the detection of novel nitrilase genes. The presented study identified partial sequences of 15 novel nitrilase genes across 7 genera, with partial DNA sequence homology (%) displayed across an additional 16 genera. This research will prove valuable in the screening of microorganisms for the identification of novel clade-specific nitrilase genes, with predicted enantioselective profiles as determined by their clade "],"journal":["MicrobiologyOpen"],"pagination":["e00700"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6460282"],"repository":["biostudies-literature"],"pubmed_title":["The use of clade-specific PCR assays to identify novel nitrilase genes from environmental isolates."],"pmcid":["PMC6460282"],"pubmed_authors":["O'Reilly C","Coffey L","Dooley-Cullinane TM","Weiner DP","O'Meara D","Owens E","Aslam B","O'Neill D"],"additional_accession":[]},"is_claimable":false,"name":"The use of clade-specific PCR assays to identify novel nitrilase genes from environmental isolates.","description":"Nitrilase enzymes (EC 3.5.5.1) are responsible for the direct hydration of nitriles to their corresponding carboxylic acids and ammonia. The utilization of nitrilase enzymes in biocatalysis toward bio-pharmaceuticals and industrial applications facilitates the move towards green chemistry. The body of research presented describes a novel clade-specific touchdown PCR protocol for the detection of novel nitrilase genes. The presented study identified partial sequences of 15 novel nitrilase genes across 7 genera, with partial DNA sequence homology (%) displayed across an additional 16 genera. This research will prove valuable in the screening of microorganisms for the identification of novel clade-specific nitrilase genes, with predicted enantioselective profiles as determined by their clade ","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Apr","modification":"2026-05-02T03:30:45.735Z","creation":"2019-06-06T22:46:23Z"},"accession":"S-EPMC6460282","cross_references":{"pubmed":["30597773"],"doi":["10.1002/mbo3.700"]}}