{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Jin BJ"],"funding":["NIBIB NIH HHS","National Eye Institute","NEI NIH HHS","National Institute of Diabetes and Digestive and Kidney Diseases","NIDDK NIH HHS","Emily's Entourage","Cystic Fibrosis Foundation","National Institute of Biomedical Imaging and Bioengineering"],"pagination":["7675-7681"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6461040"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["90(12)"],"pubmed_abstract":["New high-throughput assay formats and innovative screening technologies are needed for miniaturized screens using small quantities of near-native, patient-derived cells. Here, we developed a hollow micropillar array method to screen compounds using epithelial cells cultured on a porous support, with the goal of screening thousands of compounds using a single 24 mm diameter transwell filter containing cultured cells. Test compounds (∼1 nL) in an alginate hydrogel were printed by microinjection in hollow cylindrical micropillars (height = 150 μm, inner diameter = 100 μm) spaced 300 μm apart in a square array configuration. Compounds were delivered by positioning the array near the surface of a cell layer, with 5-10 μm of distance between the micropillars and cell surface. Micropillar array g"],"journal":["Analytical chemistry"],"pubmed_title":["Hollow Micropillar Array Method for High-Capacity Drug Screening on Filter-Grown Epithelial Cells."],"pmcid":["PMC6461040"],"funding_grant_id":["DK075302","R01 EB000415","R24 DK099803","EY13574","R01 DK075302","R01 DK101373","R01 EY013574","EB00415","R37 EB000415","DK101373","DK72517","P30 DK072517","DK099803"],"pubmed_authors":["Lee S","Verkman AS","Jin BJ"],"additional_accession":[]},"is_claimable":false,"name":"Hollow Micropillar Array Method for High-Capacity Drug Screening on Filter-Grown Epithelial Cells.","description":"New high-throughput assay formats and innovative screening technologies are needed for miniaturized screens using small quantities of near-native, patient-derived cells. Here, we developed a hollow micropillar array method to screen compounds using epithelial cells cultured on a porous support, with the goal of screening thousands of compounds using a single 24 mm diameter transwell filter containing cultured cells. Test compounds (∼1 nL) in an alginate hydrogel were printed by microinjection in hollow cylindrical micropillars (height = 150 μm, inner diameter = 100 μm) spaced 300 μm apart in a square array configuration. Compounds were delivered by positioning the array near the surface of a cell layer, with 5-10 μm of distance between the micropillars and cell surface. Micropillar array g","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Jun","modification":"2025-04-22T17:16:26.927Z","creation":"2019-06-06T21:10:27Z"},"accession":"S-EPMC6461040","cross_references":{"pubmed":["29779372"],"doi":["10.1021/acs.analchem.8b01554"]}}