biostudies-literatureJin BJNIBIB NIH HHSNational Eye InstituteNEI NIH HHSNational Institute of Diabetes and Digestive and Kidney DiseasesNIDDK NIH HHSEmily's EntourageCystic Fibrosis FoundationNational Institute of Biomedical Imaging and Bioengineering7675-7681https://www.ebi.ac.uk/biostudies/studies/S-EPMC6461040biostudies-literatureUnknown90(12)New high-throughput assay formats and innovative screening technologies are needed for miniaturized screens using small quantities of near-native, patient-derived cells. Here, we developed a hollow micropillar array method to screen compounds using epithelial cells cultured on a porous support, with the goal of screening thousands of compounds using a single 24 mm diameter transwell filter containing cultured cells. Test compounds (∼1 nL) in an alginate hydrogel were printed by microinjection in hollow cylindrical micropillars (height = 150 μm, inner diameter = 100 μm) spaced 300 μm apart in a square array configuration. Compounds were delivered by positioning the array near the surface of a cell layer, with 5-10 μm of distance between the micropillars and cell surface. Micropillar array gAnalytical chemistryHollow Micropillar Array Method for High-Capacity Drug Screening on Filter-Grown Epithelial Cells.PMC6461040DK075302R01 EB000415R24 DK099803EY13574R01 DK075302R01 DK101373R01 EY013574EB00415R37 EB000415DK101373DK72517P30 DK072517DK099803Lee SVerkman ASJin BJfalseHollow Micropillar Array Method for High-Capacity Drug Screening on Filter-Grown Epithelial Cells.New high-throughput assay formats and innovative screening technologies are needed for miniaturized screens using small quantities of near-native, patient-derived cells. Here, we developed a hollow micropillar array method to screen compounds using epithelial cells cultured on a porous support, with the goal of screening thousands of compounds using a single 24 mm diameter transwell filter containing cultured cells. Test compounds (∼1 nL) in an alginate hydrogel were printed by microinjection in hollow cylindrical micropillars (height = 150 μm, inner diameter = 100 μm) spaced 300 μm apart in a square array configuration. Compounds were delivered by positioning the array near the surface of a cell layer, with 5-10 μm of distance between the micropillars and cell surface. Micropillar array g2018-01-01T00:00:00Z2018 Jun2025-04-22T17:16:26.927Z2019-06-06T21:10:27ZS-EPMC64610402977937210.1021/acs.analchem.8b01554