biostudies-literatureUnknown11(1)Kabiri K<h4>Background and objectives</h4>Interrogation of the genomic relations between Iranian <i>Mycoplasma agalactiae</i> vaccine strains of Taliqan, Lorestan and Shiraz.<h4>Materials and methods</h4>Two MLVA (covering VNTR loci of 5, 9, 17 and 19) and MLST (comprising <i>dnaA, gltX, gyrB,</i> C, <i>tufA</i> genes) genotyping systems plus nucleotide structure analysis of P80 gene, was conducted.<h4>Results</h4>The shared MLVA pattern represented by the three strains differed to that of the <i>Mag</i> PG2 laboratory strain, only at locus VNTR19 where the PG2 genome hold a 3 bp longer stretch. In MLST analysis, at <i>dnaA, gltX, gyrB, metS</i> and <i>tufA</i> loci, the three strains displayed alleles 1, 21, 2, 2 and 1, respectively. At, <i>gltX</i> locus a new allele (21) was detected where a neIranian journal of microbiology48-54https://www.ebi.ac.uk/biostudies/studies/S-EPMC6462265biostudies-literatureHomogeneity of <i>Mycoplasma agalctiae</i> vaccine strains in an agalaxy- high-burden environment.PMC6462265Tadayon KKabiri KNorouzi JSekhavati MPourbakhsh SAfalseHomogeneity of <i>Mycoplasma agalctiae</i> vaccine strains in an agalaxy- high-burden environment.<h4>Background and objectives</h4>Interrogation of the genomic relations between Iranian <i>Mycoplasma agalactiae</i> vaccine strains of Taliqan, Lorestan and Shiraz.<h4>Materials and methods</h4>Two MLVA (covering VNTR loci of 5, 9, 17 and 19) and MLST (comprising <i>dnaA, gltX, gyrB,</i> C, <i>tufA</i> genes) genotyping systems plus nucleotide structure analysis of P80 gene, was conducted.<h4>Results</h4>The shared MLVA pattern represented by the three strains differed to that of the <i>Mag</i> PG2 laboratory strain, only at locus VNTR19 where the PG2 genome hold a 3 bp longer stretch. In MLST analysis, at <i>dnaA, gltX, gyrB, metS</i> and <i>tufA</i> loci, the three strains displayed alleles 1, 21, 2, 2 and 1, respectively. At, <i>gltX</i> locus a new allele (21) was detected where a ne2019-01-01T00:00:00Z2019 Feb2025-04-22T10:24:22.002Z2019-06-06T21:10:48ZS-EPMC646226530996831