biostudies-literatureMeng ENational Science Foundation of ChinaE257https://www.ebi.ac.uk/biostudies/studies/S-EPMC6466307biostudies-literatureUnknown11(3)The complete sequence of a novel RNA virus isolated from <i>Tetrastichus brontispae</i> (TbRV-1) was determined to be 12,239 nucleotides in length with five non-overlapping, linearly arranged coding sequences (CDS), potentially encoding nucleoproteins, hypothetical proteins, matrix proteins, glycoproteins, and RNA-dependent RNA polymerases. Sequence analysis indicated that the RNA-dependent RNA polymerase of TbRV-1 shares a 65% nucleotide and 67% amino acid sequence identity with Hubei dimarhabdovirus 2, suggesting that TbRV-1 is a member of the dimarhabdovirus supergroup. This corresponded to the result of the phylogenetic analysis. The affiliation of TbRV-1 with members of the family Rhabdoviridae was further validated by similar transcription termination motifs (GGAACUUUUUUU) to the <i>VirusesThe First Complete Genome Sequence of a Novel <i>Tetrastichus brontispae</i> RNA Virus-1 (TbRV-1).PMC646630731471829Meng EQiao THou YMTang BFu LWu JLSanchez-Garcia FJWang YChen ZMfalseThe First Complete Genome Sequence of a Novel <i>Tetrastichus brontispae</i> RNA Virus-1 (TbRV-1).The complete sequence of a novel RNA virus isolated from <i>Tetrastichus brontispae</i> (TbRV-1) was determined to be 12,239 nucleotides in length with five non-overlapping, linearly arranged coding sequences (CDS), potentially encoding nucleoproteins, hypothetical proteins, matrix proteins, glycoproteins, and RNA-dependent RNA polymerases. Sequence analysis indicated that the RNA-dependent RNA polymerase of TbRV-1 shares a 65% nucleotide and 67% amino acid sequence identity with Hubei dimarhabdovirus 2, suggesting that TbRV-1 is a member of the dimarhabdovirus supergroup. This corresponded to the result of the phylogenetic analysis. The affiliation of TbRV-1 with members of the family Rhabdoviridae was further validated by similar transcription termination motifs (GGAACUUUUUUU) to the <i>2019-01-01T00:00:00Z2019 Mar2026-04-07T18:17:45.256Z2019-06-06T21:11:34ZS-EPMC64663073087124810.3390/v11030257