biostudies-literatureOberemok VVRussian Foundation for Basic Research6197https://www.ebi.ac.uk/biostudies/studies/S-EPMC6470133biostudies-literatureUnknown9(1)Having observed how botanicals and other natural compounds are used by nature to control pests in the environment, we began investigating natural polymers, DNA and RNA, as promising tools for insect pest management. Over the last decade, unmodified short antisense DNA oligonucleotides have shown a clear potential for use as insecticides. Our research has concentrated mainly on Lymantria dispar larvae using an antisense oligoRING sequence from its inhibitor-of-apoptosis gene. In this article, we propose a novel biotechnology to protect plants from insect pests using DNA insecticide with improved insecticidal activity based on a new antisense oligoRIBO-11 sequence from the 5.8S ribosomal RNA gene. This investigational oligoRIBO-11 insecticide causes higher mortality among both L. dispar larvScientific reportsDNA insecticide developed from the Lymantria dispar 5.8S ribosomal RNA gene provides a novel biotechnology for plant protection.PMC647013318-316-00063Gal'chinsky NVDyadichev VVBessalova EYOberemok VVGninenko YIKrasnodubets AMLaikova KVNovikov IATemirova ZZShumskykh MNRepetskaya AIUseinov RZFomochkina IIMakalish TPKubyshkin AVfalseDNA insecticide developed from the Lymantria dispar 5.8S ribosomal RNA gene provides a novel biotechnology for plant protection.Having observed how botanicals and other natural compounds are used by nature to control pests in the environment, we began investigating natural polymers, DNA and RNA, as promising tools for insect pest management. Over the last decade, unmodified short antisense DNA oligonucleotides have shown a clear potential for use as insecticides. Our research has concentrated mainly on Lymantria dispar larvae using an antisense oligoRING sequence from its inhibitor-of-apoptosis gene. In this article, we propose a novel biotechnology to protect plants from insect pests using DNA insecticide with improved insecticidal activity based on a new antisense oligoRIBO-11 sequence from the 5.8S ribosomal RNA gene. This investigational oligoRIBO-11 insecticide causes higher mortality among both L. dispar larv2019-01-01T00:00:00Z2019 Apr2025-04-26T23:05:52.096Z2019-06-06T22:46:52ZS-EPMC64701333099627710.1038/s41598-019-42688-8