{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Becker M"],"funding":["Ministerium für Wissenschaft, Forschung und Kunst Baden-Württemberg"],"pagination":["76"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6470187"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["7"],"pubmed_abstract":["Biopharmaceutical production processes strive for the optimization of economic efficiency. Among others, the maximization of volumetric productivity is a key criterion. Typical parameters such as partial pressure of CO<sub>2</sub> (pCO<sub>2</sub>) and pH are known to influence the performance although reasons are not yet fully elucidated. In this study the effects of pCO<sub>2</sub> and pH shifts on the phenotypic performance were linked to metabolic and energetic changes. Short peak performance of q<sub>mAb</sub> (23 pg/cell/day) was achieved by early pCO<sub>2</sub> shifts up to 200 mbar but followed by declining intracellular ATP levels to 2.5 fmol/cell and 80% increase of q<sub>Lac</sub>. On the contrary, steadily rising q<sub>mAb</sub> could be installed by slight pH down-shifts ensu"],"journal":["Frontiers in bioengineering and biotechnology"],"pubmed_title":["The Less the Better: How Suppressed Base Addition Boosts Production of Monoclonal Antibodies With Chinese Hamster Ovary Cells."],"pmcid":["PMC6470187"],"funding_grant_id":["32-7546.31/1/4"],"pubmed_authors":["Bechmann J","Becker M","Takors R","Junghans L","Teleki A"],"additional_accession":[]},"is_claimable":false,"name":"The Less the Better: How Suppressed Base Addition Boosts Production of Monoclonal Antibodies With Chinese Hamster Ovary Cells.","description":"Biopharmaceutical production processes strive for the optimization of economic efficiency. Among others, the maximization of volumetric productivity is a key criterion. Typical parameters such as partial pressure of CO<sub>2</sub> (pCO<sub>2</sub>) and pH are known to influence the performance although reasons are not yet fully elucidated. In this study the effects of pCO<sub>2</sub> and pH shifts on the phenotypic performance were linked to metabolic and energetic changes. Short peak performance of q<sub>mAb</sub> (23 pg/cell/day) was achieved by early pCO<sub>2</sub> shifts up to 200 mbar but followed by declining intracellular ATP levels to 2.5 fmol/cell and 80% increase of q<sub>Lac</sub>. On the contrary, steadily rising q<sub>mAb</sub> could be installed by slight pH down-shifts ensu","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019","modification":"2025-04-26T23:09:41.764Z","creation":"2019-06-06T22:56:59Z"},"accession":"S-EPMC6470187","cross_references":{"pubmed":["31032253"],"doi":["10.3389/fbioe.2019.00076"]}}