biostudies-literatureBecker MMinisterium für Wissenschaft, Forschung und Kunst Baden-Württemberg76https://www.ebi.ac.uk/biostudies/studies/S-EPMC6470187biostudies-literatureUnknown7Biopharmaceutical production processes strive for the optimization of economic efficiency. Among others, the maximization of volumetric productivity is a key criterion. Typical parameters such as partial pressure of CO₂ (pCO₂) and pH are known to influence the performance although reasons are not yet fully elucidated. In this study the effects of pCO₂ and pH shifts on the phenotypic performance were linked to metabolic and energetic changes. Short peak performance of q_mAb (23 pg/cell/day) was achieved by early pCO₂ shifts up to 200 mbar but followed by declining intracellular ATP levels to 2.5 fmol/cell and 80% increase of q_Lac. On the contrary, steadily rising q_mAb could be installed by slight pH down-shifts ensuFrontiers in bioengineering and biotechnologyThe Less the Better: How Suppressed Base Addition Boosts Production of Monoclonal Antibodies With Chinese Hamster Ovary Cells.PMC647018732-7546.31/1/4Bechmann JBecker MTakors RJunghans LTeleki AfalseThe Less the Better: How Suppressed Base Addition Boosts Production of Monoclonal Antibodies With Chinese Hamster Ovary Cells.Biopharmaceutical production processes strive for the optimization of economic efficiency. Among others, the maximization of volumetric productivity is a key criterion. Typical parameters such as partial pressure of CO₂ (pCO₂) and pH are known to influence the performance although reasons are not yet fully elucidated. In this study the effects of pCO₂ and pH shifts on the phenotypic performance were linked to metabolic and energetic changes. Short peak performance of q_mAb (23 pg/cell/day) was achieved by early pCO₂ shifts up to 200 mbar but followed by declining intracellular ATP levels to 2.5 fmol/cell and 80% increase of q_Lac. On the contrary, steadily rising q_mAb could be installed by slight pH down-shifts ensu2019-01-01T00:00:00Z20192025-04-26T23:09:41.764Z2019-06-06T22:56:59ZS-EPMC64701873103225310.3389/fbioe.2019.00076