{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Grant MKO"],"funding":["NIA NIH HHS","NCI NIH HHS","NINDS NIH HHS","National Institutes of Health"],"pagination":["6"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6474067"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["21"],"pubmed_abstract":["Antibodies are commonly used to detect or isolate proteins from biological samples. Much attention has been paid to the potential for poorly-characterized antibodies to lead to misleading results, but <i>antibody-independent</i> artefacts may also occur. Here, we recount two examples of antibody-independent artefacts that have confounded the interpretation of results in our search for molecular entities associated with memory loss in Alzheimer's disease (AD). First, when using biotin-avidin systems for antibody detection, endogenous biotinylated proteins created spurious bands in Western blots of brain lysates from AD patients and transgenic mouse models of AD. These artefactual bands occurred in a transgene- and strain-dependent manner. A second, unexpected artefact occurred when Protein "],"journal":["Biological procedures online"],"pubmed_title":["A Cautionary Tale: Endogenous Biotinylated Proteins and Exogenously-Introduced Protein A Cause Antibody-Independent Artefacts in Western Blot Studies of Brain-Derived Proteins."],"pmcid":["PMC6474067"],"funding_grant_id":["RC1 AG035870","RC1-AG35870","P30 CA077598","R01-NS33249","R01 NS033249"],"pubmed_authors":["Zahs KR","Ashe KH","Liu P","Grant MKO","Shapiro SL"],"additional_accession":[]},"is_claimable":false,"name":"A Cautionary Tale: Endogenous Biotinylated Proteins and Exogenously-Introduced Protein A Cause Antibody-Independent Artefacts in Western Blot Studies of Brain-Derived Proteins.","description":"Antibodies are commonly used to detect or isolate proteins from biological samples. Much attention has been paid to the potential for poorly-characterized antibodies to lead to misleading results, but <i>antibody-independent</i> artefacts may also occur. Here, we recount two examples of antibody-independent artefacts that have confounded the interpretation of results in our search for molecular entities associated with memory loss in Alzheimer's disease (AD). First, when using biotin-avidin systems for antibody detection, endogenous biotinylated proteins created spurious bands in Western blots of brain lysates from AD patients and transgenic mouse models of AD. These artefactual bands occurred in a transgene- and strain-dependent manner. A second, unexpected artefact occurred when Protein ","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019","modification":"2026-05-01T14:02:49.315Z","creation":"2019-06-06T22:58:27Z"},"accession":"S-EPMC6474067","cross_references":{"pubmed":["31019379"],"doi":["10.1186/s12575-019-0095-z"]}}