{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Akiyama Y"],"funding":["MEXT | Japan Society for the Promotion of Science","Japan Agency for Medical Research and Development"],"pagination":["7738-7743"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6475437"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["116(16)"],"pubmed_abstract":["Cryopreservation is widely used to maintain backups of cells as it enables the semipermanent storage of cells. During the freezing process, ice crystals that are generated inside and outside the cells can lethally damage the cells. All conventional cryopreservation methods use at least one cryoprotective agent (CPA) to render water inside and outside the cells vitreous or nanocrystallized (near-vitrification) without forming damaging ice crystals. However, CPAs should ideally be avoided due to their cytotoxicity and potential side effects on the cellular state. Herein, we demonstrate the CPA-free cryopreservation of mammalian cells by ultrarapid cooling using inkjet cell printing, which we named superflash freezing (SFF). The SFF cooling rate, which was estimated by a heat-transfer stimula"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Cryoprotectant-free cryopreservation of mammalian cells by superflash freezing."],"pmcid":["PMC6475437"],"funding_grant_id":["17K19028","26709013","25560222","JP18mk0104117"],"pubmed_authors":["Akiyama Y","Watanabe H","Yamada S","Kanda Y","Shinose M"],"additional_accession":[]},"is_claimable":false,"name":"Cryoprotectant-free cryopreservation of mammalian cells by superflash freezing.","description":"Cryopreservation is widely used to maintain backups of cells as it enables the semipermanent storage of cells. During the freezing process, ice crystals that are generated inside and outside the cells can lethally damage the cells. All conventional cryopreservation methods use at least one cryoprotective agent (CPA) to render water inside and outside the cells vitreous or nanocrystallized (near-vitrification) without forming damaging ice crystals. However, CPAs should ideally be avoided due to their cytotoxicity and potential side effects on the cellular state. Herein, we demonstrate the CPA-free cryopreservation of mammalian cells by ultrarapid cooling using inkjet cell printing, which we named superflash freezing (SFF). The SFF cooling rate, which was estimated by a heat-transfer stimula","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Apr","modification":"2025-04-19T22:56:53.875Z","creation":"2019-10-11T07:01:52Z"},"accession":"S-EPMC6475437","cross_references":{"pubmed":["30936320"],"doi":["10.1073/pnas.1808645116"]}}