biostudies-literatureUnknown33(2)Kim BCDespite their potential applications in future regenerative medicine, periodontal ligament stem cells (PDLSCs) are difficult to obtain in large amounts from patients. Therefore, maintaining stemness while expanding the cell numbers for medical use is the key to transitioning PDLSCs from the bench to the clinic. Lysophosphatidic acid (LPA), which is present in the human body and saliva, is a signaling molecule derived from phospholipids. In this study, we examined the effects of LPA on stemness maintenance in human PDLSCs. Several spindle-shaped and fibroblast-like periodontal ligament stem-like cell lines were established from PDLSC isolation. Among these cell lines, the most morphologically appropriate cell line was characterized. The expression levels of OCT4, NANOG (a stem cell marker),Journal of biomedical research122-130https://www.ebi.ac.uk/biostudies/studies/S-EPMC6477173biostudies-literatureEffects of lysophosphatidic acid on human periodontal ligament stem cells from teeth extracted from dental patients.PMC6477173So KHHyun SHSong JIKim BCfalseEffects of lysophosphatidic acid on human periodontal ligament stem cells from teeth extracted from dental patients.Despite their potential applications in future regenerative medicine, periodontal ligament stem cells (PDLSCs) are difficult to obtain in large amounts from patients. Therefore, maintaining stemness while expanding the cell numbers for medical use is the key to transitioning PDLSCs from the bench to the clinic. Lysophosphatidic acid (LPA), which is present in the human body and saliva, is a signaling molecule derived from phospholipids. In this study, we examined the effects of LPA on stemness maintenance in human PDLSCs. Several spindle-shaped and fibroblast-like periodontal ligament stem-like cell lines were established from PDLSC isolation. Among these cell lines, the most morphologically appropriate cell line was characterized. The expression levels of OCT4, NANOG (a stem cell marker),2019-01-01T00:00:00Z2019 Apr2025-05-31T22:33:06.013Z2025-05-31T22:33:06.013ZS-EPMC64771733101096110.7555/JBR.32.20170123