biostudies-literatureEaston AVLondon Centre for Neglected Tropical Disease ResearchMedical Research CouncilDivision of Intramural Research, National Institute of Allergy and Infectious DiseasesNIAID Microbiome Programe00519-19https://www.ebi.ac.uk/biostudies/studies/S-EPMC6479000biostudies-literatureUnknown10(2)Murine studies suggest that the presence of some species of intestinal helminths is associated with changes in host microbiota composition and diversity. However, studies in humans have produced varied conclusions, and the impact appears to vary widely depending on the helminth species present. To demonstrate how molecular approaches to the human gut microbiome can provide insights into the complex interplay among disparate organisms, DNA was extracted from cryopreserved stools collected from residents of 5 rural Kenyan villages prior to and 3 weeks and 3 months following albendazole (ALB) therapy. Samples were analyzed by quantitative PCR (qPCR) for the presence of 8 species of intestinal parasites and by MiSeq 16S rRNA gene sequencing. Based on pretreatment results, the presence of neithmBioThe Impact of Anthelmintic Treatment on Human Gut Microbiota Based on Cross-Sectional and Pre- and Postdeworming Comparisons in Western Kenya.PMC6479000MR/R015600/1Odiere MRKepha SQuinones MEaston AVOliveira RGVujkovic-Cvijin IAnderson RMBelkaid YNutman TBfalseThe Impact of Anthelmintic Treatment on Human Gut Microbiota Based on Cross-Sectional and Pre- and Postdeworming Comparisons in Western Kenya.Murine studies suggest that the presence of some species of intestinal helminths is associated with changes in host microbiota composition and diversity. However, studies in humans have produced varied conclusions, and the impact appears to vary widely depending on the helminth species present. To demonstrate how molecular approaches to the human gut microbiome can provide insights into the complex interplay among disparate organisms, DNA was extracted from cryopreserved stools collected from residents of 5 rural Kenyan villages prior to and 3 weeks and 3 months following albendazole (ALB) therapy. Samples were analyzed by quantitative PCR (qPCR) for the presence of 8 species of intestinal parasites and by MiSeq 16S rRNA gene sequencing. Based on pretreatment results, the presence of neith2019-01-01T00:00:00Z2019 Apr2026-06-16T03:13:53.321Z2025-05-29T16:17:32.361ZS-EPMC64790003101532410.1128/mBio.00519-19