biostudies-literatureNagy ZBritish Heart FoundationMedical Research Council331-343https://www.ebi.ac.uk/biostudies/studies/S-EPMC6484457biostudies-literatureUnknown133(4)Conditional knockout (KO) mouse models are invaluable for elucidating the physiological roles of platelets. The <i>Platelet factor 4-Cre recombinase</i> (<i>Pf4-Cre</i>) transgenic mouse is the current model of choice for generating megakaryocyte/platelet-specific KO mice. Platelets and leukocytes work closely together in a wide range of disease settings, yet the specific contribution of platelets to these processes remains unclear. This is partially a result of the <i>Pf4-Cre</i> transgene being expressed in a variety of leukocyte populations. To overcome this issue, we developed a <i>Gp1ba-Cre</i> transgenic mouse strain in which <i>Cre</i> expression is driven by the endogenous <i>Gp1ba</i> locus. By crossing <i>Gp1ba-Cre</i> and <i>Pf4-Cre</i> mice to the <i>mT/mG</i> dual-fluorescenceBloodThe <i>Gp1ba-Cre</i> transgenic mouse: a new model to delineate platelet and leukocyte functions.PMC6484457FS/13/1/29894RG/15/13/31673GBT1564FS/15/58/31784Vogtle TGareus RDi Nunzio GGeer MJTarakhovsky ANeel BGDesanti GEWeiss ASenis YAMazharian AHeising SMori JNagy ZfalseThe <i>Gp1ba-Cre</i> transgenic mouse: a new model to delineate platelet and leukocyte functions.Conditional knockout (KO) mouse models are invaluable for elucidating the physiological roles of platelets. The <i>Platelet factor 4-Cre recombinase</i> (<i>Pf4-Cre</i>) transgenic mouse is the current model of choice for generating megakaryocyte/platelet-specific KO mice. Platelets and leukocytes work closely together in a wide range of disease settings, yet the specific contribution of platelets to these processes remains unclear. This is partially a result of the <i>Pf4-Cre</i> transgene being expressed in a variety of leukocyte populations. To overcome this issue, we developed a <i>Gp1ba-Cre</i> transgenic mouse strain in which <i>Cre</i> expression is driven by the endogenous <i>Gp1ba</i> locus. By crossing <i>Gp1ba-Cre</i> and <i>Pf4-Cre</i> mice to the <i>mT/mG</i> dual-fluorescence2019-01-01T00:00:00Z2019 Jan2026-04-29T01:16:59.046Z2025-05-29T19:47:28.977ZS-EPMC64844573042916110.1182/blood-2018-09-877787