biostudies-literatureColli DFAmerican Heart AssociationNHLBI NIH HHSNational Institutes of HealthNational Institute of General Medical SciencesNIGMS NIH HHS1386-1393https://www.ebi.ac.uk/biostudies/studies/S-EPMC6486484biostudies-literatureUnknown116(8)In mammalian ventricular cardiomyocytes, invaginations of the surface membrane form the transverse tubular system (T-system), which consists of transverse tubules (TTs) that align with sarcomeres and Z-lines as well as longitudinal tubules (LTs) that are present between Z-lines in some species. In many cardiac disease etiologies, the T-system is perturbed, which is believed to promote spatially heterogeneous, dyssynchronous Ca²⁺ release and inefficient contraction. In general, T-system characterization approaches have been directed primarily at isolated cells and do not detect subcellular T-system heterogeneity. Here, we present MatchedMyo, a matched-filter-based algorithm for subcellular T-system characterization in isolated cardiomyocytes and millimeter-scale myocardial sectioBiophysical journalA Matched-Filter-Based Algorithm for Subcellular Classification of T-System in Cardiac Tissues.PMC6486484P20 GM103527P30 GM127211R01 HL132067R35GM124977R35 GM124977P20GM10352717UFEL33490002Louch WESachse FBBlood SRSankarankutty ACFrisk MColli DFKekenes-Huskey PMfalseA Matched-Filter-Based Algorithm for Subcellular Classification of T-System in Cardiac Tissues.In mammalian ventricular cardiomyocytes, invaginations of the surface membrane form the transverse tubular system (T-system), which consists of transverse tubules (TTs) that align with sarcomeres and Z-lines as well as longitudinal tubules (LTs) that are present between Z-lines in some species. In many cardiac disease etiologies, the T-system is perturbed, which is believed to promote spatially heterogeneous, dyssynchronous Ca²⁺ release and inefficient contraction. In general, T-system characterization approaches have been directed primarily at isolated cells and do not detect subcellular T-system heterogeneity. Here, we present MatchedMyo, a matched-filter-based algorithm for subcellular T-system characterization in isolated cardiomyocytes and millimeter-scale myocardial sectio2019-01-01T00:00:00Z2019 Apr2026-06-04T02:17:06.2Z2021-02-21T08:49:38ZS-EPMC64864843097955310.1016/j.bpj.2019.03.010