biostudies-literatureUnknown2Maity PPCollagen II (COLII), the most abundant protein in vertebrates, helps maintain the structural and functional integrity of cartilage. Delivery of COLII from animal sources could improve cartilage regeneration therapies. Here we show that COLII can be purified from the Capra ear cartilage, a commonly available bio-waste product, with a high yield. MALDI-MS/MS analysis evidenced post-translational modifications of the signature triplet, Glycine-Proline-Hydroxyproline (G-P-Hyp), in alpha chain of isolated COLII (COLIIA1). Additionally, thirty-two peptides containing 59 Hyp residues and a few G-X-Y triplets with positional alterations of Hyp in COLIIA1 are also identified. Furthermore, we show that an injectable hydrogel formulation containing the isolated COLII facilitates chondrogenic differenCommunications biology146https://www.ebi.ac.uk/biostudies/studies/S-EPMC6488623biostudies-literatureIsolation and mass spectrometry based hydroxyproline mapping of type II collagen derived from <i>Capra hircus</i> ear cartilage.PMC6488623Das AKDatta PDutta DGanguly SDhara SChowdhury ARDixit KSamanta RKapat KDas NCMaity PPfalseIsolation and mass spectrometry based hydroxyproline mapping of type II collagen derived from <i>Capra hircus</i> ear cartilage.Collagen II (COLII), the most abundant protein in vertebrates, helps maintain the structural and functional integrity of cartilage. Delivery of COLII from animal sources could improve cartilage regeneration therapies. Here we show that COLII can be purified from the Capra ear cartilage, a commonly available bio-waste product, with a high yield. MALDI-MS/MS analysis evidenced post-translational modifications of the signature triplet, Glycine-Proline-Hydroxyproline (G-P-Hyp), in alpha chain of isolated COLII (COLIIA1). Additionally, thirty-two peptides containing 59 Hyp residues and a few G-X-Y triplets with positional alterations of Hyp in COLIIA1 are also identified. Furthermore, we show that an injectable hydrogel formulation containing the isolated COLII facilitates chondrogenic differen2019-01-01T00:00:00Z20192026-05-01T08:55:00.904Z2019-06-06T22:50:27ZS-EPMC64886233104417110.1038/s42003-019-0394-6