biostudies-literatureCreff ACNRSEuropean Research CouncilANRRégion Auvergne-Rhône-AlpesMinistère de l’Enseignement Supérieur, de la Recherche Scientifique et des Technologies de l'Information et de la Communicatione1007847https://www.ebi.ac.uk/biostudies/studies/S-EPMC6490923biostudies-literatureUnknown15(4)The embryonic cuticle is necessary for normal seed development and seedling establishment in Arabidopsis. Although mutants with defective embryonic cuticles have been identified, neither the deposition of cuticle material, nor its regulation, has been described during embryogenesis. Here we use electron microscopy, cuticle staining and permeability assays to show that cuticle deposition initiates de novo in patches on globular embryos. By combining these techniques with genetics and gene expression analysis, we show that successful patch coalescence to form a continuous cuticle requires a signalling involving the endosperm-specific subtilisin protease ALE1 and the receptor kinases GSO1 and GSO2, which are expressed in the developing embryonic epidermis. Transcriptome analysis shows that thPLoS geneticsA stress-response-related inter-compartmental signalling pathway regulates embryonic cuticle integrity in Arabidopsis.PMC6490923#307387MetaboHUB-ANR-11-INBS-0010Doctoral Grant307387ANR-13-BSV2-0002Domergue FBrocard LDoll NMPascal SMoussu SJoubes JMarsollier ACGalletti RWidiez TBoeuf STaconnat LIngram GCreff AfalseA stress-response-related inter-compartmental signalling pathway regulates embryonic cuticle integrity in Arabidopsis.The embryonic cuticle is necessary for normal seed development and seedling establishment in Arabidopsis. Although mutants with defective embryonic cuticles have been identified, neither the deposition of cuticle material, nor its regulation, has been described during embryogenesis. Here we use electron microscopy, cuticle staining and permeability assays to show that cuticle deposition initiates de novo in patches on globular embryos. By combining these techniques with genetics and gene expression analysis, we show that successful patch coalescence to form a continuous cuticle requires a signalling involving the endosperm-specific subtilisin protease ALE1 and the receptor kinases GSO1 and GSO2, which are expressed in the developing embryonic epidermis. Transcriptome analysis shows that th2019-01-01T00:00:00Z2019 Apr2026-04-07T13:54:11.703Z2019-06-06T23:25:45ZS-EPMC64909233099868410.1371/journal.pgen.1007847