biostudies-literatureChen LLDepartment of Science and Technology of Zhejiang ProvinceHangzhou Science and Technology Major Project for InnovationProject of Experiment Animal PlatformNational Natural Science Foundation of ChinaHangzhou Scientific Research Foundation for Returned Scholars1000-1008https://www.ebi.ac.uk/biostudies/studies/S-EPMC6492873biostudies-literatureUnknown22(12)<h4>Aims</h4>Previous study suggests that mTOR signaling pathway may play an important role in epileptogenesis. The present work was designed to explore the contribution of raptor protein to the development of epilepsy and comorbidities.<h4>Methods</h4>Mice with conditional knockout of raptor protein were generated by cross-bred Rptor^flox/flox mice with nestin-CRE mice. The expression of raptor protein was analyzed by Western blotting in brain tissue samples. Neuronal death and mossy fiber sprouting were detected by FJB staining and Timm staining, respectively. Spontaneous seizures were recorded by EEG-video system. Morris water maze, open field test, and excitability test were used to study the behaviors of Rptor CKO mice.<h4>Results</h4>As the consequence of deleting Rptor, doCNS neuroscience & therapeuticsNeural Progenitor Cells Rptor Ablation Impairs Development but Benefits to Seizure-Induced Behavioral Abnormalities.PMC649287320152013A0220142013C3702681371429Dong JYZhu FWu XMChen LLWu MLKai JJZeng LHfalseNeural Progenitor Cells Rptor Ablation Impairs Development but Benefits to Seizure-Induced Behavioral Abnormalities.<h4>Aims</h4>Previous study suggests that mTOR signaling pathway may play an important role in epileptogenesis. The present work was designed to explore the contribution of raptor protein to the development of epilepsy and comorbidities.<h4>Methods</h4>Mice with conditional knockout of raptor protein were generated by cross-bred Rptor^flox/flox mice with nestin-CRE mice. The expression of raptor protein was analyzed by Western blotting in brain tissue samples. Neuronal death and mossy fiber sprouting were detected by FJB staining and Timm staining, respectively. Spontaneous seizures were recorded by EEG-video system. Morris water maze, open field test, and excitability test were used to study the behaviors of Rptor CKO mice.<h4>Results</h4>As the consequence of deleting Rptor, do2016-01-01T00:00:00Z2016 Dec2025-04-26T23:16:42.649Z2019-07-25T07:14:49ZS-EPMC64928732767724810.1111/cns.12607