{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Pouokam M"],"funding":["U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences","NIGMS NIH HHS","National Science Foundation"],"pagination":["6795"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6494875"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(1)"],"pubmed_abstract":["The three dimensional organization of genomes remains mostly unknown due to their high degree of condensation. Biophysical studies predict that condensation promotes the topological entanglement of chromatin fibers and the inhibition of function. How organisms balance between functionally active genomes and a high degree of condensation remains to be determined. Here we hypothesize that the Rabl configuration, characterized by the attachment of centromeres and telomeres to the nuclear envelope, helps to reduce the topological entanglement of chromosomes. To test this hypothesis we developed a novel method to quantify chromosome entanglement complexity in 3D reconstructions obtained from Chromosome Conformation Capture (CCC) data. Applying this method to published data of the yeast genome, we show that computational models implementing the attachment of telomeres or centromeres alone are not sufficient to obtain the reduced entanglement complexity observed in 3D reconstructions. It is only when the centromeres and telomeres are attached to the nuclear envelope (i.e. the Rabl configuration) that the complexity of entanglement of the genome is comparable to that of the 3D reconstructions. We therefore suggest that the Rabl configuration is an essential player in the simplification of the entanglement of chromatin fibers."],"journal":["Scientific reports"],"pubmed_title":["The Rabl configuration limits topological entanglement of chromosomes in budding yeast."],"pmcid":["PMC6494875"],"funding_grant_id":["DMS1716987","R01 GM109457","GM109457","DMS1817156","R01 GM075119","GM 109457"],"pubmed_authors":["Pouokam M","Arsuaga J","Cruz B","Segal MR","Burgess S","Vazquez M"],"additional_accession":[]},"is_claimable":false,"name":"The Rabl configuration limits topological entanglement of chromosomes in budding yeast.","description":"The three dimensional organization of genomes remains mostly unknown due to their high degree of condensation. Biophysical studies predict that condensation promotes the topological entanglement of chromatin fibers and the inhibition of function. How organisms balance between functionally active genomes and a high degree of condensation remains to be determined. Here we hypothesize that the Rabl configuration, characterized by the attachment of centromeres and telomeres to the nuclear envelope, helps to reduce the topological entanglement of chromosomes. To test this hypothesis we developed a novel method to quantify chromosome entanglement complexity in 3D reconstructions obtained from Chromosome Conformation Capture (CCC) data. Applying this method to published data of the yeast genome, we show that computational models implementing the attachment of telomeres or centromeres alone are not sufficient to obtain the reduced entanglement complexity observed in 3D reconstructions. It is only when the centromeres and telomeres are attached to the nuclear envelope (i.e. the Rabl configuration) that the complexity of entanglement of the genome is comparable to that of the 3D reconstructions. We therefore suggest that the Rabl configuration is an essential player in the simplification of the entanglement of chromatin fibers.","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 May","modification":"2025-04-21T23:59:35.781Z","creation":"2019-06-06T23:26:11Z"},"accession":"S-EPMC6494875","cross_references":{"pubmed":["31043625"],"doi":["10.1038/s41598-019-42967-4"]}}