{"database":"biostudies-literature","file_versions":[],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":62,"searchCount":0},"additional":{"submitter":["Pullmannova P"],"funding":["Grantová Agentura České Republiky","Joint Institute for Nuclear Research","Efficiency and Safety Improvement of Current Drugs and Nutraceuticals: Advanced Methods-New Challenges","European Regional Development Fund"],"pagination":["963-971"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6495169"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["60(5)"],"pubmed_abstract":["Membrane models of the stratum corneum (SC) lipid barrier, either healthy or affected by recessive X-linked ichthyosis, constructed from ceramide [Cer; nonhydroxyacyl sphingosine <i>N</i>-tetracosanoyl-d-<i>erythro</i>-sphingosine (CerNS24) alone or with omega-<i>O-</i>acylceramide <i>N</i>-(32-linoleyloxy)dotriacontanoyl-d-<i>erythro</i>-sphingosine (CerEOS)], FFAs(C16-24), cholesterol (Chol), and sodium cholesteryl sulfate (CholS) were investigated. X-ray diffraction (XRD) revealed a previously unreported polymorphism of the membranes. In the absence of CerEOS, the membranes formed a short lamellar phase (SLP; the repeat distance <i>d</i> = 5.3 nm), a medium lamellar phase (MLP; <i>d</i> = 10.6 nm), or very long lamellar phases (VLLP; <i>d</i> = 15.9 and 21.2 nm). An increased CholS-to-Chol ratio modulated the membrane polymorphism, although the CholS phase separated at ≥ 7 weight% (of total lipids). The presence of CerEOS led to the stable long lamellar phase (LLP) with <i>d</i> = 12.2 nm and prevented VLLP formation. Our XRD results agree well with recently published cryo-electron microscopy data for vitreous skin sections, while also revealing new structures. Thus, lamellar phases with long repeat distances (MLP and VLLP) may be formed in the absence of omega-<i>O-</i>acylceramide, whereas these ultralong Cer species likely stabilize the final SC lipid architecture of LLP by riveting the adjacent lipid layers."],"journal":["Journal of lipid research"],"pubmed_title":["Long and very long lamellar phases in model stratum corneum lipid membranes."],"pmcid":["PMC6495169"],"funding_grant_id":["19-09135J"],"pubmed_authors":["Zbytovska J","Vavrova K","Pullmannova P","Maixner J","Kucerka N","Ermakova E","Opalka L","Kovacik A"],"view_count":["62"],"additional_accession":[]},"is_claimable":false,"name":"Long and very long lamellar phases in model stratum corneum lipid membranes.","description":"Membrane models of the stratum corneum (SC) lipid barrier, either healthy or affected by recessive X-linked ichthyosis, constructed from ceramide [Cer; nonhydroxyacyl sphingosine <i>N</i>-tetracosanoyl-d-<i>erythro</i>-sphingosine (CerNS24) alone or with omega-<i>O-</i>acylceramide <i>N</i>-(32-linoleyloxy)dotriacontanoyl-d-<i>erythro</i>-sphingosine (CerEOS)], FFAs(C16-24), cholesterol (Chol), and sodium cholesteryl sulfate (CholS) were investigated. X-ray diffraction (XRD) revealed a previously unreported polymorphism of the membranes. In the absence of CerEOS, the membranes formed a short lamellar phase (SLP; the repeat distance <i>d</i> = 5.3 nm), a medium lamellar phase (MLP; <i>d</i> = 10.6 nm), or very long lamellar phases (VLLP; <i>d</i> = 15.9 and 21.2 nm). An increased CholS-to-Chol ratio modulated the membrane polymorphism, although the CholS phase separated at ≥ 7 weight% (of total lipids). The presence of CerEOS led to the stable long lamellar phase (LLP) with <i>d</i> = 12.2 nm and prevented VLLP formation. Our XRD results agree well with recently published cryo-electron microscopy data for vitreous skin sections, while also revealing new structures. Thus, lamellar phases with long repeat distances (MLP and VLLP) may be formed in the absence of omega-<i>O-</i>acylceramide, whereas these ultralong Cer species likely stabilize the final SC lipid architecture of LLP by riveting the adjacent lipid layers.","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 May","modification":"2024-02-14T23:14:00.286Z","creation":"2021-02-20T17:48:08Z"},"accession":"S-EPMC6495169","cross_references":{"pubmed":["30885924"],"doi":["10.1194/jlr.m090977","10.1194/jlr.M090977"]}}